SH3 Domain-Containing Protein 2 Plays a Crucial Role at the Step of Membrane Tubulation during Cell Plate Formation
SH3 Domain-Containing Protein 2 Plays a Crucial Role at the Step of Membrane Tubulation during Cell Plate Formation
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DOI：
10.1105/tpc.17.00108
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发表时间：
2017-06-01
期刊：
影响因子：
11.6
通讯作者：
Hwang, Inhwan
中科院分区：
文献类型：
--
作者：
Ahn, Gyeongik;Kim, Hyeran;Hwang, Inhwan
在植物胞质分裂过程中，高尔基体网络衍生的小泡聚集在分裂细胞的中心，并经历各种结构变化以产生平面细胞板。然而，这种转换如何在分子水平上发生仍然是难以捉摸的。在这项研究中，我们报告，SH3结构域包含蛋白2（SH3P2）在拟南芥中起着至关重要的作用，在转换囊泡的平面细胞板。SH3P2 RNAi植物表现出分裂缺陷型，并在细胞板的前缘产生囊泡聚集。SH3P2定位于细胞板的前缘，特别是细胞板的收缩或弯曲区域。SH3P2的BAR结构域可诱导囊泡的微管化。SH3P2与DRP1A形成复合物，影响DRP1A向细胞板的聚集。基于这些结果，我们建议，SH3P2功能与DRP1A一起将融合的囊泡转化为管状结构在胞质分裂。
During cytokinesis in plants, trans-Golgi network-derived vesicles accumulate at the center of dividing cells and undergo various structural changes to give rise to the planar cell plate. However, how this conversion occurs at the molecular level remains elusive. In this study, we report that SH3 Domain-Containing Protein 2 (SH3P2) in Arabidopsis thaliana plays a crucial role in converting vesicles to the planar cell plate. SH3P2 RNAi plants showed cytokinesis-defective phenotypes and produced aggregations of vesicles at the leading edge of the cell plate. SH3P2 localized to the leading edge of the cell plate, particularly the constricted or curved regions of the cell plate. The BAR domain of SH3P2 induced tubulation of vesicles. SH3P2 formed a complex with dynamin-related protein 1A (DRP1A) and affected DRP1A accumulation to the cell plate. Based on these results, we propose that SH3P2 functions together with DRP1A to convert the fused vesicles to tubular structures during cytokinesis.