MALDI imaging of lipids after matrix sublimation/deposition
MALDI imaging of lipids after matrix sublimation/deposition
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DOI：
10.1016/j.bbalip.2011.04.012
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发表时间：
2011-11-01
影响因子：
4.8
通讯作者：
Berry, Karin A. Zemski
中科院分区：
文献类型：
--
作者：
Murphy, Robert C.;Hankin, Joseph A.;Berry, Karin A. Zemski
已经开发了质谱技术来记录生物分子的质谱，包括脂质，因为它们天然存在于组织内，从而允许产生显示组织、器官和完整动物中特定脂质分布的图像。这些技术基于基质辅助激光解吸/电离（MALDI），需要在分析前将基质应用于组织表面。最近显示出对于脂质分析的显著优点的一种应用技术是基质的升华，然后直接气相沉积到组织上。提出了通过升华/沉积实现的与激光脉冲后的样品温度和基质晶体尺寸相关的增强灵敏度的解释。升华/沉积的各种器官，包括脑，眼组织和肾脏的脂质成像的具体例子。这篇文章是一个特殊问题的一部分，题为：脂肪组学和成像质谱。(C)2011 Elsevier B. V.保留所有权利。
Mass spectrometric techniques have been developed to record mass spectra of biomolecules including lipids as they naturally exist within tissues and thereby permit the generation of images displaying the distribution of specific lipids in tissues, organs, and intact animals. These techniques are based on matrix-assisted laser desorption/ionization (MALDI) that requires matrix application onto the tissue surface prior to analysis. One technique of application that has recently shown significant advantages for lipid analysis is sublimation of matrix followed by vapor deposition directly onto the tissue. Explanations for enhanced sensitivity realized by sublimation/deposition related to sample temperature after a laser pulse and matrix crystal size are presented. Specific examples of sublimation/deposition in lipid imaging of various organs including brain, ocular tissue, and kidney are presented. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrom. (C) 2011 Elsevier B.V. All rights reserved.