Effects of Chromosomal Integration of the Vitreoscilla Hemoglobin Gene (vgb) and S-Adenosylmethionine Synthetase Gene (metK) on ε-Poly-L-Lysine Synthesis in Streptomyces albulus NK660
Effects of Chromosomal Integration of the Vitreoscilla Hemoglobin Gene (vgb) and S-Adenosylmethionine Synthetase Gene (metK) on ε-Poly-L-Lysine Synthesis in Streptomyces albulus NK660
复制标题
玻璃颤菌血红蛋白基因 (vgb) 和 S-腺苷甲硫氨酸合成酶基因 (metK) 染色体整合对白色链霉菌 NK660 ε-聚-L-赖氨酸合成的影响
DOI：
10.1007/s12010-015-1958-7
复制
发表时间：
2016-04-01
影响因子：
3
通讯作者：
Song, Cunjiang
中科院分区：
文献类型：
--
作者：
Gu, Yanyan;Wang, Xiaomeng;Song, Cunjiang
ε-聚赖氨酸（epsilon-PL）是一种广泛应用的天然食品防腐剂。为了检测透明质酸血红蛋白（VHb）和S-腺苷甲硫氨酸（SAM）对白色链霉菌NK 660合成ε-磷脂酶的影响，将外源VHb基因（vgb）和SAM合成酶基因（metK）分别插入到白色链霉菌NK 660中。在组成型ermE* 启动子控制下的白蛋白NK 660染色体。CO差谱分析表明S. albulus NK 660-VHb株能表达功能性VHb。S. albulus NK 660-VHb产生的ε-PL和生物量分别比野生型对照高26.67%和14.57%。反相高压液相色谱（RP-HPLC）结果表明，metK基因的过表达导致S. albulus NK 660-SAM的表达，使菌体生物量增加，并使epsilon-PL合成酶基因（pls）的转录水平提高。结果表明，vgb和metK基因的表达提高了S. albulusNK660。
epsilon-Poly-l-lysine (epsilon-PL) is a widely used natural food preservative. To test the effects of the Vitreoscilla hemoglobin (VHb) and S-adenosylmethionine (SAM) on epsilon-PL synthesis in Streptomyces albulus NK660, the heterologous VHb gene (vgb) and SAM synthetase gene (metK) were inserted into the S. albulus NK660 chromosome under the control of the constitutive ermE* promoter. CO-difference spectrum analysis showed S. albulus NK660-VHb strain could express functional VHb. S. albulus NK660-VHb produced 26.67 % higher epsilon-PL and 14.57 % higher biomass than the wild-type control, respectively. Reversed-phase high-pressure liquid chromatography (RP-HPLC) results showed the overexpression of the metK gene resulted in increased intracellular SAM synthesis in S. albulus NK660-SAM, which caused increases of biomass as well as the transcription level of epsilon-PL synthetase gene (pls). Results indicated that the expression of vgb and metK gene improved on epsilon-PL synthesis and biomass for S. albulus NK660, respectively.