Interactions between the unicellular red alga Rhodella reticulata (Rhodophyta) and contaminated bacteria
Interactions between the unicellular red alga Rhodella reticulata (Rhodophyta) and contaminated bacteria
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单细胞红藻红藻（Rhodophyta）与污染细菌之间的相互作用
DOI：
10.1046/j.1365-2672.2002.01717.x
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发表时间：
2002
影响因子：
4
通讯作者：
J. Ivanova
中科院分区：
文献类型：
--
作者：
T. Toncheva;J. Ivanova
目的：明确细菌菌株LR1和LR3在细胞吞噬杆菌LR2引起的罗德拉细胞破坏中的作用。
方法和结果：细菌是从破坏的藻类培养中获得的。在纯培养中分离出它们，并用Polymmicrotest检测其生化活性。研究了细菌对藻类多糖的降解和利用能力。这些细菌是在以罗德拉多糖为唯一碳源的培养基中培养的。测定培养基中还原糖的含量。利用扫描电子显微镜(SEM)对网纹红假单胞菌(Rhodella ReNetata)进行了细菌定位研究。LR2。
结论：网纹红假单胞菌的裂解是这3株细菌与原病原菌Cytophaga sp.共同作用的结果。LR2起主要作用。菌株LR1和LR3的多糖和代谢产物的积累促进了细胞吞噬菌的发育。LR2。该菌株对特定藻类培养条件的适应和以多糖为唯一碳源的利用支持了其在藻类悬浮液中的稳定生长和对罗德拉细胞的破坏。
研究的意义和影响：Cytophaga sp.的优势LR2超过其他两种污染物和网纹红藻细胞的裂解是由于细菌附着在藻类多糖鞘上的能力造成的。粘液和挤出物的形成促进了细菌在藻类表面的黏附现象以及聚集性藻类-细菌球体的形成。这些聚集体的沉积降低了藻类菌株的光合作用能力，导致细胞裂解，最终导致罗德拉死亡。
Aims: To define the role of the bacterial strains LR1 and LR3 in the Rhodella cell destruction caused by Cytophaga sp.LR2.
Methods and Results: The bacteria were obtained from algal culture with destruction. They were isolated in pure culture and tested for biochemical activities using Polymicrotest. The ability of bacteria to degrade and utilize the algal polysaccharide was investigated. The bacteria were grown in a media containing Rhodella polysaccharide as a sole carbon source. The level of the reducing sugars in the culture media was determined. Scanning electron microscopy (SEM) was used to define the location of bacteria in extensively and intensively cultivated Rhodella reticulata previously infected by Cytophaga sp. LR2.
Conclusions: The lysis of Rhodella reticulata cells is due to the joint action of the three bacterial strains with the former pathogen Cytophaga sp. LR2 playing the main role. The accumulation of the polysaccharide and the excreted metabolites of the strains LR1 and LR3 stimulated the development of Cytophaga sp. LR2. The adaptation of the strain to particular conditions of alga cultivation and the utilization of polysaccharide as a sole carbon source supported its stable growth in alga suspension and destruction of Rhodella cells.
Significance and Impact of the Study: The predominance of Cytophaga sp. LR2 over the two other contaminants and the lysis of Rhodella reticulata cells resulted from the ability of the bacterium to attach to the algal polysaccharide sheath. The formation of slime and extrusions facilitated the phenomenon of bacterial adhesion to the algal surface as well as the formation of colonial alga – bacterial spherules. The sedimentation of these aggregates decreased the ability of the algal strain to photosynthesize, led to the lysis of the cells and finally caused the death of Rhodella.