Developmental stages of the Japanese quail
Developmental stages of the Japanese quail
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DOI：
10.1111/j.1469-7580.2009.01173.x
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Evans, Darrell J. R.
中科院分区：
文献类型：
--
作者：
Ainsworth, Sophie J.;Stanley, Rachael L.;Evans, Darrell J. R.
发育生物学研究以各种鸟类作为模式生物来研究形态发生，其中以鸡胚为主要类群。对鸡胚胎的关注使汉堡和汉密尔顿在近60年前发展了他们的最终分期系列，这个系列仍然是所有从事禽类胚胎研究的实验室的支柱。对小鸡胚胎的关注在某种程度上掩盖了另一种鸟类胚胎的重要性，这种鸟类胚胎已被证明同样强大，那就是日本鹌鹑。自20世纪60年代末以来，用小鸡和鹌鹑的胚胎制造了嵌合体，这项技术彻底改变了研究发育过程中细胞和分子相互作用的方法。对文献的回顾表明，许多研究小组正在以许多既定的和新的方式使用鹌鹑胚胎，并且该物种已成为发育生物学的主要动物模型。已经对鹌鹑进行了一些分期，但这是不完整的，而且描述、阶段和孵化时间的变化意味着与雏鸟的比较并不总是容易的。人们似乎普遍认为，在胚胎发生的早期阶段，小鸡和鹌鹑的胚胎在发育上几乎没有区别，尽管这一观点的基础尚未在实验中建立起来。在发育的中后期，鹌鹑胚胎的加速个体发育意味着与雏鸟的登记丢失了。因此，我们为日本鹌鹑开发了一个明确的发育阶段系列，以便充分表征差异，避免误解或假设，并且比较研究的结果不会被扭曲。
Developmental biology research has used various avian species as model organisms for studying morphogenesis, with the chick embryo being used by the majority of groups. The focus on the chick embryo led Hamburger and Hamilton to develop their definitive staging series nearly 60 years ago and this series is still the mainstay of all laboratories working with avian embryos. The focus on the chick embryo has somewhat overshadowed the importance of another avian embryo that has proved to be equally powerful, the Japanese quail. Since the late 1960s, chimeras have been produced using chick and quail embryos and this technique has revolutionized the approach taken to the investigation of the cellular and molecular interactions that occur during development. Reviews of the literature demonstrate that many research groups are using the quail embryo in a number of established and new ways, and this species has become a primary animal model in developmental biology. Some staging of quail has been performed but this has been incomplete and variations in descriptions, stages and incubation timings mean that comparisons with the chick are not always easily made. There appears to be general agreement that, at the early stages of embryogenesis, there is little developmental difference between chick and quail embryos, although the basis for this has not been established experimentally. The accelerated ontogeny of quail embryos at mid to late stages of development means that registration with the chick is lost. We have therefore developed a definitive developmental stage series for Japanese quail so that differences are fully characterized, misconceptions or assumptions are avoided, and the results of comparative studies are not distorted.