Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure
Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure
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DOI：
10.1016/j.bbrc.2008.07.037
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发表时间：
2008-09-19
影响因子：
3.1
通讯作者：
Ohta, Toshiko
中科院分区：
文献类型：
--
作者：
Kuroda, Makoto;Tanaka, Yoshikazu;Ohta, Toshiko
众所周知，金黄色葡萄球菌在人体皮肤上定殖，其中生理条件的特征在于高变的水活性，即，反复脱水或再水化。为了确定在高变水活度下定殖的促进因素，我们研究了巨大的蛋白Ebh（细胞外基质（ECM）结合蛋白同源物）。ebh突变体RAM 8显示沿隔膜沿着内陷的液泡，类似于在部分质体中发现的，并且细胞在渗透压升高下破裂。RAM 8对突然高渗升高、替考拉宁和Triton X-100也相对敏感。通过使用绿色荧光蛋白（GFP）作为报告基因，Ebh定位于整个细胞表面。这表明Ebh可能通过在细胞壁和细胞质膜之间形成一个桥梁，以避免在高渗条件下的质体破坏，从而有助于结构的稳态。(C)2008年爱思唯尔公司All rights reserved.
Staphylococcus aureus is well known to colonize on human skin where the physiological condition is characterized by hypervariable water activity, i.e., repeated dehydration or rehydration. To determine the facilitating factors for the colonization under hypervariable water activity, we studied the giant Protein Ebh (extracellular matrix (ECM)-binding protein homologue). The ebh mutant RAM8 showed invaginated vacuoles along the septum, similar to that found in partial plasmolysis, and the cells burst under osmotic upshift. RAM8 was also relatively susceptible to abrupt hyperosmotic upshift, teicoplanin, and Triton X-100. By using the green fluorescent protein (GFP) as a reporter, Ebh was localized over the entire cell Surface. This suggests that Ebh might contribute to structural homeostasis by forming a bridge between the cell-wall and cytoplasmic membrane to avoid plasmolysis under hyperosmotic condition. (C) 2008 Elsevier Inc. All rights reserved.