Enhanced fluorescence signal in nonlinear microscopy through supplementary fiber-optic light collection
Enhanced fluorescence signal in nonlinear microscopy through supplementary fiber-optic light collection
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DOI：
10.1364/oe.17.006421
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发表时间：
2009-04-13
期刊：
影响因子：
3.8
通讯作者：
Helmchen, Fritjof
中科院分区：
文献类型：
--
作者：
Engelbrecht, Christoph J.;Goebel, Werner;Helmchen, Fritjof
非线性显微技术关键依赖于有效的信号检测。在这里，我们提出了一个大芯光纤环，用于收集荧光光子，这些光子没有通过物镜传输，因此通常会被浪费。理论处理表明，这种辅助光纤光收集系统(SUFICS)可以提供高达4倍的信号增益。在典型的活体成像实验中，光纤环通道在800微米深度内比目标通道更亮，因此提供了增益>；2。此外，SUFICS将钙成像实验中的噪声水平降低了约23%。我们推荐SUFICS作为一种普遍适用的、有效的非线性显微镜附件，用于增强荧光信号。(C)2009年美国光学学会
Nonlinear microscopy techniques crucially rely on efficient signal detection. Here, we present a ring of large-core optical fibers for epicollection of fluorescence photons that are not transmitted through the objective and thus normally wasted. Theoretical treatments indicated that such a supplementary fiber-optic light collection system (SUFICS) can provide an up to 4-fold signal gain. In typical in vivo imaging experiments, the fiber-ring channel was brighter than the objective channel down to 800 mu m depth, thus providing a gain >2. Moreover, SUFICS reduced noise levels in calcium imaging experiments by about 23%. We recommend SUFICS as a generally applicable, effective add-on to nonlinear microscopes for enhancing fluorescence signals. (C) 2009 Optical Society of America