Yeast β-D-glucans induced antimicrobial peptide expressions against Salmonella infection in broiler chickens
Yeast β-D-glucans induced antimicrobial peptide expressions against Salmonella infection in broiler chickens
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DOI：
10.1016/j.ijbiomac.2016.01.031
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发表时间：
2016-04-01
影响因子：
8.2
通讯作者：
Zhang, Haibo
中科院分区：
文献类型：
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作者：
Shao, Yujing;Wang, Zhong;Zhang, Haibo
本研究旨在研究酵母β-D-葡聚糖（YG）对沙门氏菌（SE）感染肉鸡内源性β-防御素（AvBDs）、组织蛋白酶抑制剂（Cath）和肝脏表达抗菌肽-2（LEAP-2）基因表达的影响。将240只1日龄Cobb肉公鸡随机分配到2 × 2因子处理组，其中包括两个水平的日粮YG（0或200 mg/kg）和两个水平的SE攻击（7-9日龄时0或1 × 10（9）SE）。结果表明，与未感染鸡相比，感染鸡的生长性能下降，盲肠移殖率和内脏器官侵入率增加，肠道特异性伊加和血清特异性IgG抗体浓度升高。SE攻毒对肉鸡空肠和脾脏中AvBD、Caths和LEAP-2基因表达的影响。但YG可抑制SE对小鼠的生长抑制作用，并进一步提高血清特异性IgG和肠道特异性伊加抗体水平。YG可降低SE感染鸡体内较高水平的沙门氏菌定植和内脏器官侵入。YG抑制或改变SE诱导的AMP基因差异表达模式。结果表明，YG增强了雏鸡对沙门氏菌感染的抵抗力。(C)© 2016 Elsevier B. V.版权所有。
The present study was designed to investigate the effects of yeast beta-D-glucans (YG) on gene expression of endogenous beta-defensins (AvBDs), cathelicidins (Cath) and liver-expressed antimicrobial peptide-2 (LEAP-2) in broilers challenged with Salmonella enteritidis (SE). 240 day-old Cobb male broilers were randomly assigned to 2 x 2 factorial arrangements of treatments with two levels of dietary YG (0 or 200 mg/kg in diet) and two levels of SE challenge (0 or 1 x 10(9) SE at 7-9 days of age). The results showed SE infection reduced growth performance,and increased salmonella cecal colonization and internal organs invasion, increased concentration of intestinal specific IgA and serum specific IgG antibody, as compared to uninfected birds. SE challenge differentially regulated AvBDs, Caths and LEAP-2 gene expression in the jejunum and spleen of broiler chickens during the infection period. However, YG supplementation inhibited the growth depression by SE challenge, and further increased level of serum specific IgG and intestinal specific IgA antibody. Higher level of salmonella colonization and internal organs invasion in the SE-infected birds were reduced by YG. SE-induced differentially expression patterns of AMPs genes was inhibited or changed by YG. Results indicated YG enhance chicken's resistance to salmonella infection. (C) 2016 Elsevier B.V. All rights reserved.