Comparison of 20nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages
Comparison of 20nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages
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DOI：
10.1116/1.4926547
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发表时间：
2015-09-01
期刊：
影响因子：
2.1
通讯作者：
Ryan, Mary P.
中科院分区：
文献类型：
--
作者：
Munusamy, Prabhakaran;Wang, Chongmin;Ryan, Mary P.
银纳米粒子的广泛使用引发了环境和生物影响的问题。许多合成方法用于生产针对特定应用优化的纯银和银壳金核颗粒。由于纳米颗粒和从颗粒中溶解的银都可能影响生物学反应，因此了解不同工艺产生的纳米颗粒的物理化学特性沿着生物学影响非常重要。作者研究了以不同方式合成的两种20 nm银颗粒的结构、溶解和颗粒暴露于巨噬细胞的影响，这两种颗粒具有不同的内部结构。通过电子显微镜检查结构，并在含有10%胎牛血清的Rosewell Park Memorial Institute培养基中测定溶出度。使用细胞毒性和氧化应激来测量对RAW 264.7巨噬细胞的生物学影响。颗粒是多晶的，但是在金种子颗粒上生长的20 nm颗粒具有较小的微晶尺寸，具有许多高能晶界和缺陷，并且比20 nm纯银颗粒具有明显更高的溶解度。对于含有Au核的20 nm颗粒，观察到比20 nm纯银颗粒更大的氧化应激和细胞毒性。一个简单的溶解模型描述了粒径的时间变化和溶解的银的颗粒负载大于9 μ g/ml的24小时内的许多体外研究的特点。(c)2015年作者。
Widespread use of silver nanoparticles raises questions of environmental and biological impact. Many synthesis approaches are used to produce pure silver and silver-shell gold-core particles optimized for specific applications. Since both nanoparticles and silver dissolved from the particles may impact the biological response, it is important to understand the physicochemical characteristics along with the biological impact of nanoparticles produced by different processes. The authors have examined the structure, dissolution, and impact of particle exposure to macrophage cells of two 20 nm silver particles synthesized in different ways, which have different internal structures. The structures were examined by electron microscopy and dissolution measured in Rosewell Park Memorial Institute media with 10% fetal bovine serum. Cytotoxicity and oxidative stress were used to measure biological impact on RAW 264.7 macrophage cells. The particles were polycrystalline, but 20 nm particles grown on gold seed particles had smaller crystallite size with many high-energy grain boundaries and defects, and an apparent higher solubility than 20 nm pure silver particles. Greater oxidative stress and cytotoxicity were observed for 20 nm particles containing the Au core than for 20 nm pure silver particles. A simple dissolution model described the time variation of particle size and dissolved silver for particle loadings larger than 9 mu g/ml for the 24-h period characteristic of many in-vitro studies. (c) 2015 Author(s).