Transient inhibition of translation initiation by osmotic stress
Transient inhibition of translation initiation by osmotic stress
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DOI：
10.1074/jbc.m108848200
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发表时间：
2002-04-19
影响因子：
4.8
通讯作者：
Toh-e, A
中科院分区：
文献类型：
--
作者：
Uesono, Y;Toh-e, A
细胞会对环境的变化做出反应和适应。在这项研究中，我们研究了环境应激对酵母囊蛋白质合成的影响。酿酒酵母我们发现，渗透胁迫导致蛋氨酸摄取的不可逆抑制，尿嘧啶摄取的瞬时抑制，葡萄糖摄取的瞬时刺激，核糖体蛋白（RP）基因如CYH 2和RPS 27的瞬时抑制，以及翻译起始的瞬时抑制。通过渗透胁迫快速抑制翻译起始需要一种新的途径，该途径不同于氨基酸敏感途径、葡萄糖敏感途径和TOR途径。Hog1 MAP激酶途径不参与抑制甲硫氨酸摄取或翻译起始，但需要适应翻译起始后抑制和抑制RP基因的渗透胁迫。这些结果表明，翻译起始的瞬时抑制是由Hog1通路对翻译的急性抑制和翻译的长期激活共同作用的结果。
Cells respond and adapt to changes in the environment. In this study, we examined the effect of environmental stresses on protein synthesis in the yeast Sac. charomyces cerevisiae. We found that osmotic stress causes irreversible inhibition of methionine uptake, transient inhibition of uracil uptake, transient stimulation of glucose uptake, transient repression of ribosomal protein (RP) genes such as CYH2 and RPS27, and the transient inhibition of translation initiation. Rapid inhibition of translation initiation by osmotic stress requires a novel pathway, different from the amino acid-sensing pathway, the glucose-sensing pathway, and the TOR pathway. The Hog1 MAP kinase pathway is not involved in the inhibition of either methionine uptake or translation initiation but is required for the adaptation of translation initiation after inhibition and the repression of RP genes by osmotic stress. These results suggest that the transient inhibition of translation initiation occurs as a result of a combination of both acute inhibition of translation and the long-term activation of translation by the Hog1 pathway.