Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa
Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa
复制标题
DOI：
10.1007/s00774-004-0550-y
复制
发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Katsube, K
中科院分区：
文献类型：
--
作者：
Kawashima, N;Shindo, K;Katsube, K
用股骨骨髓基质细胞和其他类型间充质祖细胞建立了小鼠成骨祖细胞库萨细胞系。我们从几个方面对Kusa的两个亚系（Kusa- a1和Kusa- o）进行了表征，包括使用表达谱系统，cDNA微阵列。原Kusa亚系（Kusa- a1）在诱导矿化的条件下具有高碱性磷酸酶活性和高钙沉积积累，具有抗坏血酸和β -甘油磷酸。Kusa- o是Kusa的低成骨亚系，在诱导条件下碱性磷酸酶活性高，但钙沉积积累缓慢。这两个库萨亚系在矿化过程中成骨标志基因骨钙素和骨桥蛋白的表达存在差异。一种cDNA芯片显示，在诱导条件下，Kusa-A1和Kusa-O的基因表达均显著下调。采用另一种高通量芯片检测Kusa-A1和Kusa-O基因表达模式的差异。通过这一分析，骨膜蛋白，这将参与成骨的一个阶段，是低的Kusa-A1。相反，肌细胞增强因子2C (Myocyte enhancer factor 2C, MEF2C)，一种肌生成转录因子，在kasa - a1中高表达，尽管没有任何其他肌生成基因的表达。
A cell line of murine osteogenic progenitor cells, Kusa, was established from femoral bone marrow stromal cells with other types of mesenchymal progenitor cells. We characterized two sublines of Kusa (Kusa-A1 and Kusa-O) from several aspects, including the use of an expression profiling system, a cDNA microarray. The original Kusa subline (Kusa-A1) had high alkaline phosphatase activity and high accumulation of calcium deposits in a condition inducing mineralization, with ascorbic acid and beta-glycerophosphate. Kusa-O, a low osteogenic subline of Kusa, had high alkaline phosphatase activity but slow accumulation of calcium deposits even in the inducing condition. These two Kusa sublines differed in the expression of the osteogenic marker genes, osteocalcin and osteopontin, during mineralization. A type of cDNA microarray revealed marked downregulation of gene expression in the inducing condition in both Kusa-A1 and Kusa-O. Another type of high-throughput microarray was performed to examine the difference in gene expression patterns between Kusa-A1 and Kusa-O. By this analysis, periostin, which would be involved in a stage of osteogenesis, was low in Kusa-A1. On the contrary, Myocyte enhancer factor 2C (MEF2C), a myogenic transcriptional factor, was high in Kusa-A1, although no expression of any other myogenic genes was shown.