Subcellular localization of VDAC in mitochondria and ER in the cerebellum
Subcellular localization of VDAC in mitochondria and ER in the cerebellum
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DOI：
10.1016/j.bbabio.2004.02.009
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发表时间：
2004-07-09
影响因子：
4.3
通讯作者：
Vardi, N
中科院分区：
文献类型：
--
作者：
Shoshan-Barmatz, V;Zalk, R;Vardi, N
电压依赖性阴离子通道（VDAC）提供腺嘌呤核苷酸、Ca2+和其他代谢物进出线粒体的通道。在这里，VDAC在脑线粒体和ER中的细胞内定位和寡聚组织被证明。免疫组织化学染色显示小脑中的VDAC高标记的浦肯野神经元。小脑分子层的免疫金标记和EM分析显示，线粒体外膜的特异性VDAC免疫染色，在线粒体之间或线粒体与相关ER之间的接触部位高度增强。纯化的ER膜含有VDAC，但不含其他线粒体蛋白。分离的线粒体、ER或纯化的VDAC的化学交联证明了VDAC以寡聚体形式存在。基于富集VDAC的交界面密切相关的线粒体和ER膜和VDAC低聚物的存在，我们提出了一个专门的线粒体或ER和线粒体之间的膜间通信VDAC的参与，以补充这些细胞器之间观察到的紧密的结构和功能耦合。(C)2004 Elsevier B.V保留所有权利。
The voltage-dependent anion channel (VDAC) provides passage for adenine nucleotides, Ca2+ and other metabolites into and from mitochondria. Here, the intracellular localization and oligomeric organization of VDAC in brain mitochondria and ER are demonstrated. Immunohistochemical staining of VDAC in rat cerebellum showed high labeling of the Purkinje neurons. Immunogold labeling and EM analysis of the cerebellar molecular layer showed specific VDAC immunostaining of the mitochondrial outer membrane, highly enhanced in contact sites between mitochondria or between mitochondria and associated ER. Purified ER membranes contain VDAC, but not other mitochondrial proteins. Chemical cross-linking of isolated mitochondria, ER or purified VDAC demonstrated the existence of VDAC in oligomeric form. Based on the enrichment of VDAC in the junctional face of closely associated mitochondrial and ER membranes and the existence of VDAC oligomers, we propose an involvement of VDAC in specialized intermembrane communication between mitochondria or between ER and mitochondria, serving to complement the tight structural and functional coupling observed between these organelles. (C) 2004 Elsevier B.V All rights reserved.