Natural ligand of mouse CD1d1: Cellular glycosylphosphatidylinositol
Natural ligand of mouse CD1d1: Cellular glycosylphosphatidylinositol
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DOI：
10.1126/science.279.5356.1541
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发表时间：
1998-03-06
期刊：
影响因子：
56.9
通讯作者：
Brutkiewicz, RR
中科院分区：
文献类型：
--
作者：
Joyce, S;Woods, AS;Brutkiewicz, RR
小鼠CD1d1是进化上保守的主要组织相容性抗原样分子CD1家族的成员，它控制着T淋巴细胞亚群NK1(+)自然T细胞的分化和功能，被认为是调节免疫反应的细胞。CD1d1晶体结构显示一个大的疏水结合位点被一个未知化学性质的配体占据。用质谱仪和代谢放射性标记鉴定细胞糖基磷脂酰肌醇是CD1d1的主要天然配体。CD1d1通过其磷脂酰肌醇高亲和力结合糖基磷脂酰肌醇。糖基磷脂酰肌醇或其他糖脂可能是CD1d1限制性T细胞的候选天然配体。
Mouse CD1d1, a member of the CD1 family of evolutionarily conserved major histocompatibility antigen-like molecules, controls the differentiation and function of a T lymphocyte subset, NK1(+) natural T cells, proposed to regulate immune responses. The CD1d1 crystal structure revealed a large hydrophobic binding site occupied by a ligand of unknown chemical nature. Mass spectrometry and metabolic radiolabeling were used to identify cellular glycosylphosphatidylinositol as a major natural ligand of CD1d1. CD1d1 bound glycosylphosphatidylinositol through its phosphatidylinositol aspect with high affinity. Glycosylphosphatidylinositol or another glycolipid could be a candidate natural ligand for CD1d1-restricted T cells.