Opioid receptor and peptide mRNA expression in proliferative zones of fetal rat central nervous system
Opioid receptor and peptide mRNA expression in proliferative zones of fetal rat central nervous system
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DOI：
10.1139/cjpp-76-3-284
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发表时间：
1998-03-01
影响因子：
2.1
通讯作者：
Winzer-Serhan, UH
中科院分区：
文献类型：
--
作者：
Leslie, FM;Chen, YL;Winzer-Serhan, UH
越来越多的证据表明阿片肽作为生长调节剂可能具有广泛的作用。为了评价内源性阿片类物质在神经发育过程中控制细胞增殖的假设，我们采用原位杂交技术研究了胚胎大鼠脑和脊髓神经上皮区阿片肽和受体mRNA的表达。我们的数据表明，前脑啡肽原mRNA广泛表达于前脑灰质区和脉络丛在妊娠后半期。相反，前强啡肽mRNA的表达仅限于腹侧脊髓的脑室周围区域。在出生前的神经元增殖的任何区域都检测到小mu或delta受体mRNA表达。然而，kappa受体mRNA在妊娠第3周广泛表达于后脑生发区。我们目前的研究结果支持这一假设，即内源性阿片类物质可以调节中枢神经系统发育过程中神经元和非神经元细胞的增殖。前脑神经上皮中前脑啡肽原mRNA和后脑内κ受体mRNA的分离表达，不同的阿片机制可能调节头端和尾侧脑区的细胞分裂。
There is increasing evidence to suggest that opioid peptides may have widespread effects as regulators of growth. To evaluate the hypothesis that endogenous opioids control cellular proliferation during neural development, we have used in situ hybridization to examine opioid peptide and receptor mRNA expression in neuroepithelial zones of fetal rat brain and spinal cord. Our data show that proenkephalin mRNA is widely expressed in forebrain grerminal zones and choroid plexus during the second half of gestation. In contrast, prodynorphin mRNA expression is restricted to the periventricular region of the ventral spinal cord. Little mu or delta receptor mRNA expression was detected in any regions of neuronal proliferation prior to birth. However, kappa receptor mRNA is widely expressed in hindbrain germinal zones during the 3rd week of gestation. Our present findings support the hypothesis that endogenous opioids ma; regulate proliferation of both neuronal and non-neuronal cells during central nervous system development. Given the segregated expression of proenkephalin mRNA in forebrain neuroepithelium and kappa receptor mRNA within hindbrain, different opioid mechanisms may regulate cell division in rostral and caudal brain regions.