Natural and antibody-dependent cell-mediated activity against Salmonella typhimurium by peripheral and intestinal lymphoid cells in mice.
Natural and antibody-dependent cell-mediated activity against Salmonella typhimurium by peripheral and intestinal lymphoid cells in mice.
复制标题
小鼠外周和肠道淋巴细胞对鼠伤寒沙门氏菌的自然和抗体依赖性细胞介导活性。
作者：
L. Nencioni;L. Villa;Diana Boraschi;B. Berti;N. A. Tagliabue;R. A. Against
用2小时的体外细胞毒性试验评估细胞介导的免疫反应。观察到GALT和外周淋巴器官的淋巴细胞具有自然的抗菌活性，而巨噬细胞则不具有这种自发活性。这种新发现的自然活性的分布是PPL大于MNL大于IEL=SPL=PBL大于POL；这与NK细胞对YAC-1肿瘤细胞的杀伤活性的器官分布无关，PBL大于SPL=IEL大于MNL=POL=PPL。此外，自然抗鼠伤寒沙门氏菌的脾效应细胞的表型与NK细胞的表型也有一定的差异。事实上，这两种细胞都是asialo GM1+、Fc受体+、非黏附性和非吞噬细胞，但前者是Thy-1.2-，后者是Thy-1.2+。Peyer‘s斑块的天然抗菌效应细胞表型与脾相同。随后观察到，加入抗鼠伤寒沙门氏菌的免疫抗体可增强其抗菌活性。使用IEL、SPL和PBL作为效应细胞，这一点尤其明显，而PPL和MNL没有显示出任何抗体依赖的抗菌活性。此外，后两个种群不能调节ADCC对CRBC的作用。采用选择性方法去除细胞群，我们观察到，至少在脾水平上，也有一种细胞，其表型特征与介导自然抗菌活性的细胞不同，但在抗体依赖反应中发挥作用。总之，这些结果表明，自然的和抗体依赖的抗菌机制在防御鼠伤寒沙门氏菌方面可能很重要，特别是在胃肠道层面，许多细菌感染首先发生在那里，并开始与宿主免疫系统相互作用。
Cell-mediated immune responses were assessed employing a 2-hr in vitro cytotoxicity assay against S. typhimurium. It was observed that lymphocytes from GALT as well as from peripheral lymphoid organs possessed natural antibacterial activity, whereas macrophages were devoid of this spontaneous activity. The distribution of this newly described natural activity was PPL greater than MnL greater than IEL = SpL = PBL greater than PoL; this did not correlate with the organ distribution of NK activity against YAC-1 tumor cells, which was PBL greater than SpL = IEL greater than MnL = PoL = PPL. Moreover, the phenotype of the splenic effector cell of the natural activity against S. typhimurium showed some differences from that of NK activity. In fact, both these cells were asialo GM1+, Fc-receptor+, nonadherent, and nonphagocytic, but the former was Thy-1.2- and the latter Thy-1.2+. The effector cell of the natural antibacterial activity in the Peyer's patches had the same phenotype as the splenic one. It was then observed that the antibacterial activity could be augmented by the addition of immune antibodies against S. typhimurium. This was particularly evident employing IEL, SpL, and PBL as effector cells, whereas PPL and MnL did not show any antibody-dependent antibacterial activity. Furthermore, these last two populations could not mediate ADCC against CRBC. Employing selective methods to deplete cell populations, we observed that, at least at the splenic level, there is also a cell that differs in its phenotypic characteristics from that mediating natural antibacterial activity but that plays a role in the antibody-dependent reactions. In conclusion, these results suggest that natural and antibody-dependent antibacterial mechanisms might be important in defense against S. typhimurium, particularly at the gastrointestinal level, where many bacterial infections first take place and begin to interact with the host immune system.