BLEOMYCIN RESISTANCE - A NEW DOMINANT SELECTABLE MARKER FOR PLANT-CELL TRANSFORMATION
BLEOMYCIN RESISTANCE - A NEW DOMINANT SELECTABLE MARKER FOR PLANT-CELL TRANSFORMATION
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DOI：
10.1007/bf00021328
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发表时间：
1986-01-01
影响因子：
5.1
通讯作者：
ZABEL, P
中科院分区：
文献类型：
--
作者：
HILLE, J;VERHEGGEN, F;ZABEL, P
植物细胞对抗生素博来霉素敏感，博来霉素是一种破坏DNA的糖肽。博来霉素抗性决定子位于转座子Tn5上，在细菌中有功能，已被克隆到植物表达载体中，并使用根癌农杆菌将其引入到烟草中。该决定子在植物细胞中的表达赋予对博来霉素的抗性，并允许选择转化的植物细胞。
Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.