Characterization of the guinea pig adipocyte thyrotropin receptor.
Characterization of the guinea pig adipocyte thyrotropin receptor.
复制标题
豚鼠脂肪细胞促甲状腺素受体的表征。
DOI：
10.1016/0006-291x(86)90964-2
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发表时间：
1986
影响因子：
3.1
通讯作者：
Nayfeh,SN
中科院分区：
文献类型：
--
作者：
Gennick,SE;ThomasJr,CG;Nayfeh,SN
~(125)I-TSH与猪甲状腺和豚鼠脂肪结合后，其高亲和力结合组分和低亲和力结合组分的解离常数相近。来自Graves病患者血清的抗体抑制了与这两个组织的高亲和力和低亲和力结合成分的结合。~(125)I-TSH与各组织膜的共价交联可导致两条蛋白条带的特异性标记。豚鼠脂肪受体亚基的MR值为52,000和38,000，而猪甲状腺受体亚基的MR值为46,000和35,000。受体亚基的标记通过与Graves自身抗体预孵育而被抑制。尽管具有不同的亚基组成，但这些组织的受体对TSH表现出相似的亲和力，并共享类似的Graves自身抗体的抗原决定簇。
125I-TSH binding to porcine thyroid and guinea pig fat resulted in curvilinear Scatchard plots with similar dissociation constants for the high and low affinity binding components. Antibodies from the sera of patients with Graves' disease inhibited binding to the high and low affinity binding components of both tissues. Covalent cross-linking of125I-TSH to membranes from each tissue resulted in the specific labeling of two protein bands. The guinea pig fat receptor subunits have Mrvalues of 52,000 and 38,000, whereas the porcine thyroid receptor subunits have values of 46,000 & 35,000. The labeling of the receptor subunits was inhibited by preincubation with Graves' autoantibodies. Despite possessing a different subunit composition, the receptors from these tissues exhibit similar affinity for TSH and share similar antigenic determinants for Graves' autoantibodies.