Fast and simple droplet sampling of sap from plant tissues and capillary microextraction of soluble saccharides for picogram-scale quantitative determination with GC-MS.
Fast and simple droplet sampling of sap from plant tissues and capillary microextraction of soluble saccharides for picogram-scale quantitative determination with GC-MS.
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快速、简单地从植物组织中对汁液进行液滴取样，并对可溶性糖进行毛细管微萃取，通过 GC-MS 进行皮克级定量测定。
DOI：
10.1021/jf102053h
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发表时间：
2010
影响因子：
6.1
通讯作者：
Xiangning Jiang
中科院分区：
文献类型：
--
作者：
Yupeng Tan;Ke Li;Lei Hu;Shu Chen;Y. Gai;Xiangning Jiang
可溶性糖是生命周期中非常重要的代谢产物，是合成结构多糖成分（纤维素、半纤维素、果胶等）的重要组成部分。细胞壁。建立了一种新的生物组织汁液液滴采样方法和毛细管操作程序，用于皮克级的气相色谱-质谱（GC-MS）定量分析。用含有核糖醇（内标）的玻璃毛细管对5至10微升树液进行取样。随后，分析物与乙酸酐乙酰化和1-甲基咪唑催化。最后用GC-MS SIM（选择离子监测）模式对可溶性生物碱进行定性检测。方法的线性范围为1×10 - 6 mol/L，理论最低检测限（LOD，s/n≥3）为1×10 - 9 mol/L。该方法适用于农业、食品科学、生物科学甚至医学研究等广泛领域的新鲜组织和其他水样中可溶性单糖的分析。
Soluble saccharides are very important metabolites of the life cycle and synthesis of structural polysaccharide components (cellulose, hemicellulose, pectin, etc.) of cell walls. A new method for droplet sampling of saps from tissues of organisms and manipulation routines in capillaries for extraction, derivation, and partitioning were developed for picogram-scale quantitative determination with gas chromatography-mass spectrometry (GC-MS). Five to ten microliters of sap was sampled with a glass capillary containing ribitol (internal standard). Subsequently, the analytes were acetylated with acetic anhydride and catalyzed by 1-methylimidazole. Finally, the soluble saccharides were qualitatively detected with GC-MS SIM (selective ion monitoring) mode. The linear ranges of the method were up to 1×10(-6) mol/L and the theoretically lowest limits of detection (LOD, s/n≥3) were up to 1×10(-9) mol/L. The method is suitable and applicable to analysis of soluble monosaccharides in fresh tissues and other aqueous samples in wide fields of agriculture, food science, biological sciences, and even medical studies.