The pel genes of the Pseudomonas aeruginosa PAK strain are involved at early and late stages of biofilm formation.
The pel genes of the Pseudomonas aeruginosa PAK strain are involved at early and late stages of biofilm formation.
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DOI：
10.1099/mic.0.27410-0
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发表时间：
2005-03
期刊：
影响因子：
1.5
通讯作者：
Perrine Vasseur;Isabelle Vallet-Gely;Chantal Soscia;S. Genin;A. Filloux
中科院分区：
文献类型：
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作者：
Perrine Vasseur;Isabelle Vallet-Gely;Chantal Soscia;S. Genin;A. Filloux
铜绿假单胞菌是一种革兰氏阴性菌，与医院感染和囊性纤维化有关。慢性细菌感染越来越多地与生物膜生活方式相关，其中小菌落嵌入细胞外基质中。用于鉴定生物膜缺陷菌株的筛选程序允许表征参与该过程的几个关键决定因素。表征了在称为pel的七基因簇中受影响的生物膜缺陷型铜绿假单胞菌PAK菌株。pel基因编码的蛋白质与多糖生物合成中涉及的组分相似，其中PelF是推定的糖基转移酶。PelG也被鉴定为多糖转运蛋白（PST）家族的推定组分。pel基因先前在铜绿假单胞菌PA14菌株中被鉴定为生产参与厚表膜和抗性生物膜形成的富含葡萄糖的基质材料所需的。然而，在PA14中，pel突变体在附着的起始阶段没有明确的表型。显示PAK菌株中的pel突变对生物膜起始几乎没有影响，但如PA14中一样，似乎产生最不稳健和成熟的生物膜。引人注目的是，通过在非纤毛铜绿假单胞菌PAK菌株中构建pel突变体，发现了pel突变在生物膜形成早期的意想不到的效果，因为观察到这些突变体在固体表面上的附着过程中严重缺陷。pel基因簇在其他革兰氏阴性细菌中是保守的，并且青枯雷尔氏菌pelG同源物ragG中的突变导致粘附缺陷。
Pseudomonas aeruginosa is a Gram-negative bacterium associated with nosocomial infections and cystic fibrosis. Chronic bacterial infections are increasingly associated with the biofilm lifestyle in which microcolonies are embedded in an extracellular matrix. Screening procedures for identifying biofilm-deficient strains have allowed the characterization of several key determinants involved in this process. Biofilm-deficient P. aeruginosa PAK strains affected in a seven-gene cluster called pel were characterized. The pel genes encode proteins with similarity to components involved in polysaccharide biogenesis, of which PelF is a putative glycosyltransferase. PelG was also identified as a putative component of the polysaccharide transporter (PST) family. The pel genes were previously identified in the P. aeruginosa PA14 strain as required for the production of a glucose-rich matrix material involved in the formation of a thick pellicle and resistant biofilm. However, in PA14, the pel mutants have no clear phenotype in the initiation phase of attachment. It was shown that pel mutations in the PAK strain had little influence on biofilm initiation but, as in PA14, appeared to generate the least robust and mature biofilms. Strikingly, by constructing pel mutants in a non-piliated P. aeruginosa PAK strain, an unexpected effect of the pel mutation in the early phase of biofilm formation was discovered, since it was observed that these mutants were severely defective in the attachment process on solid surfaces. The pel gene cluster is conserved in other Gram-negative bacteria, and mutation in a Ralstonia solanacearum pelG homologue, ragG, led to an adherence defect.