In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: serum is not essential
In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: serum is not essential
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DOI：
10.1016/s0093-691x(02)01080-4
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发表时间：
2002-12-01
期刊：
影响因子：
2.8
通讯作者：
Durrant, BS
中科院分区：
文献类型：
--
作者：
Bolamba, D;Russ, KD;Durrant, BS
用合成输卵管液（SOF）培养液评价腔前卵泡卵母细胞的体外成熟能力。晚期腔前卵泡（直径约210 μ m），并分配到四个处理组之一：（1）SOF（n = 230）;（2）SOF + 3 mg/ml牛血清白蛋白（n = 230）;（3）SOF + 20%胎牛血清（+FBS，n = 227）;或（4）SOF + 3 mg/ml BSA +20%FBS（+BSA+FBS，n = 232），然后培养长达72 h。一组对照卵泡未培养（n = 103）。在每个培养期，处理组之间达到中期I至中期II阶段（MI至MII）的卵母细胞百分比无差异。在治疗中，在MI至MII阶段的卵母细胞的百分比没有不同的培养时间。SOF组培养48 h和72 h时MI至MII期卵母细胞的比例分别为10.0%和12.2%，与对照组（0.97%）相比，差异有显著性（P < 0.05）。在+BSA组（10.1%）和+FBS组（9.7%）中，MI至MII期的卵母细胞百分比仅在培养72 μ l后才高于对照值（P < 0.05）。与对照组相比，+BSA+FBS组中MI至MII的卵母细胞百分比没有显著增加（在培养24、48和72 h时分别为3.9%、6.6%和7.6%）。这些结果表明，在所描述的条件下，培养基中添加BSA或FBS不是必需的，并且简单培养基SOF可以支持小部分母狗卵母细胞的体外核成熟。(C)2002年爱思唯尔科技有限公司All rights reserved.
The ability of oocytes from preantral follicles to mature in vitro was assessed using a synthetic oviduct fluid (SOF) medium. Advanced preantral follicles (approximately 210 pm diameter) were isolated from the ovaries of domestic bitches and assigned to one of four treatment groups: (1) SOF (n = 230); (2) SOF + 3 mg/ml bovine serum albumin (+BSA, n = 220); (3) SOF + 20% fetal bovine serum (+FBS, n = 227); or (4) SOF + 3 mg/ml BSA + 20% FBS (+BSA+FBS, n = 232), then cultured for up to 72 h. A group of control follicles was not cultured (n = 103). The percentages of oocytes reaching metaphase I to metaphase II stages (MI to MII) did not differ between treatments at each culture period. Within treatments, the percentages of oocytes at MI to MII stages did not differ with duration of culture. However, when compared to the control group (0.97%) the percentages of oocytes at MI to MII increased (P < 0.05) in the SOF group after 48 h (10.0%) and 72 h (12.2%) of culture. In the +BSA (10.1%) and +FBS (9.7%) groups, the percentages of oocytes at MI to MII increased (P < 0.05) above control values only after 72 It of culture. The percentage of oocytes at MI to MII did not significantly increase in the +BSA+FBS group (3.9, 6.6 and 7.6% at 24,48 and 72 h of culture, respectively) compared to the control group. These results indicate that under the described conditions supplementation of culture medium with BSA or FBS is not essential, and the simple medium SOF can support nuclear maturation of a small proportion of bitch oocytes in vitro. (C) 2002 Elsevier Science Inc. All rights reserved.