Ultraspiracle: An invertebrate nuclear receptor for juvenile hormones
Ultraspiracle: An invertebrate nuclear receptor for juvenile hormones
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DOI：
10.1073/pnas.94.25.13499
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发表时间：
1997-12-09
影响因子：
11.1
通讯作者：
Sharp, PA
中科院分区：
文献类型：
--
作者：
Jones, G;Sharp, PA
幼体激素（JH）是一种调节昆虫发育转变的倍半萜类配体，与核受体超胞体（USP）结合。在基于荧光的结合试验中，USP蛋白特异性结合jhiii和jhiii酸，对每个配体采用不同的最终构象状态。JH III处理表达LexA-USP融合蛋白的酿酒酵母稳定了含有该蛋白的寡聚结合，通过形成蛋白质- dna复合物检测到，并在报告基因试验中诱导usp依赖性转录。我们认为无脊椎动物形态发生转变的调控涉及JH或JH样结构与USP的结合。
Juvenile hormones (JH), a sesquiterpenoid group of ligands that regulate developmental transitions in insects, bind to the nuclear receptor ultraspiracle (USP). In fluorescence-based binding assays, USP protein binds JH III and JH III acid with specificity, adopting for each ligand a different final conformational state. JH III treatment of Saccharomyces cerevisiae expressing a LexA-USP fusion protein stabilizes an oligomeric association containing this protein, as detected by formation of a protein-DNA complex, and induces USP-dependent transcription in a reporter assay. We propose that regulation of morphogenetic transitions In invertebrates involves binding of JH or JH-like structures to USP.