Galactan-binding antibodies. Diversity and structure of idiotypes.
Galactan-binding antibodies. Diversity and structure of idiotypes.
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半乳糖结合抗体。白痴的多样性和结构。
DOI：
10.1084/jem.158.5.1385
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发表时间：
1983-11-01
影响因子：
15.3
通讯作者：
Potter, M
中科院分区：
文献类型：
--
作者：
Rudikoff, S;Pawlita, M;Pumphrey, J;Mushinski, E;Potter, M
用含β（1，6）-D-半乳聚糖的抗原诱导的一组8种IgM杂交瘤蛋白质已在一级氨基酸序列和独特型表达方面进行了表征。H链氨基酸序列显示在VH区段中非常强的同源性，尽管观察到几个取代，表明在这些IgM分子中发生体细胞突变。在CDR-3（由D片段产生的区域）和两个重组事件VH-D和D-JH中观察到显著的序列变异。发现该区域中由D片段贡献的氨基酸数量从2到6不等，然而CDR-3的总长度通过在重组过程中在D的任一侧添加氨基酸而精确地保持。这些额外的氨基酸被认为是由修复酶的核苷酸添加引起的。这些蛋白的独特型分析，结合H链序列的评估，已经允许鉴定这些分子表达的交叉反应和独特独特型决定簇的分子基础。
A group of eight IgM hybridoma proteins induced with beta(1,6)-D- galactan-containing antigens has been characterized in terms of primary amino acid sequence and idiotype expression. The H chain amino acid sequences reveal very strong homology in the VH segment although several substitutions are seen that suggest the occurrence of somatic mutation in these IgM molecules. Significant sequence variation was observed in CDR-3, the region generated by the D segment, and the two recombination events, VH-D and D-JH. The number of amino acids in this region contributed by the D segment was found to vary from two to six, yet the overall length of CDR-3 was precisely maintained by the addition of amino acids on either side of D during the recombination processes. These additional amino acids are suggested to result from nucleotide addition by repair enzymes. Idiotypic analysis of these proteins, in conjunction with an assessment of the H chain sequences, has permitted an identification of the molecular basis of both cross- reacting and unique idiotypic determinants expressed by these molecules.