ARREST AND METASTASIS OF BLOOD-BORNE TUMOR-CELLS ARE MODIFIED BY FUSION OF PLASMA-MEMBRANE VESICLES FROM HIGHLY METASTATIC CELLS
ARREST AND METASTASIS OF BLOOD-BORNE TUMOR-CELLS ARE MODIFIED BY FUSION OF PLASMA-MEMBRANE VESICLES FROM HIGHLY METASTATIC CELLS
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DOI：
10.1073/pnas.77.1.399
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
NICOLSON, GL
中科院分区：
文献类型：
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作者：
POSTE, G;NICOLSON, GL
培养的B16小鼠黑色素瘤亚系自发脱落完整的质膜囊泡。这些囊泡可以融合的细胞质膜同源和异源B16亚系使用聚乙二醇和植物血凝素P。融合的囊泡从一个高度转移的亚系（F10），专门定位在肺与细胞从一个转移性差的亚系（F1）显着增加的能力F1细胞在肺中被捕，并在这个器官中形成转移。F1囊泡与F10细胞的融合并不改变囊泡修饰的细胞在肺中定位或形成肺转移的能力。F10囊泡修饰的F1细胞在从质膜去除F10囊泡组分后恢复到其原始的阻滞行为和转移能力。F10囊泡修饰的F1细胞的阻滞和转移行为的变化具有高度特异性。来自其他转移性差且在肺中显示有限定位的B16亚系（F1、F1Lr和F10Lr）的囊泡没有改变F1细胞的阻滞行为和转移能力。显然，F1和F10亚系在肺中定位的能力的差异是由细胞表面性质的差异决定的。
B16 mouse melanoma sublines in culture spontaneously shed intact plasma membrane vesicles. These vesicles can be fused with the plasma membrane of cells from homologous and heterologous B16 sublines using polyethylene glycol and phytohemagglutinin P. Fusion of vesicles from a highly metastatic subline (F10) that localizes exclusively in the lung with cells from a poorly metastatic subline (F1) significantly increased the ability of F1 cells to become arrested in the lung and form metastases in this organ. Fusion of F1 vesicles with F10 cells did not alter the ability of vesicle-modified cells to localize in the lung or form lung metastases. F10 vesicle-modified F1 cells reverted to their original arrest behavior and metastatic capacity after removal of F10 vesicle components from the plasma membrane. The changes in the arrest and metastatic behavior of F10 vesicle-modified F1 cells were highly specific. Vesicles from other B16 sublines that are poorly metastatic and show limited localization in the lung (F1, F1Lr and F10Lr) did not modify the arrest behavior and metastatic capacity of F1 cells. Apparently the differences in the abilities of the F1 and F10 sublines to localize in the lung are determined by differences in cell surface properties.