Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves
Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves
复制标题
未成熟叶离体培养再生花生苗
作者：
L. Mroginski;K. Kartha;J. Shyluk
以3 ~ 5日龄花生幼叶为材料，研究了幼叶芽、芽和根的离体再生。CV.科罗拉多Manfredi）幼苗在确定的营养、激素和环境条件下进行了研究。将从无菌发芽的种子中取出的前两片叶（2-5 mm长）培养在含有维生素的Murashige和Skoog培养基上，如B5培养基和0.8%琼脂，补充有萘乙酸（NAA）（0.01 - 4 mg/L）和苄基腺嘌呤（BA）（1和3 mg/L）的12种组合。 所有激素组合均能产生芽，但当NAA和BA浓度均为1 mg/L时，芽再生数量最多。 虽然芽再生是最大的2至5毫米长的小叶，也获得了一些成功与小叶8-13毫米长。 将再生的芽转移到含有0.01mg/LNAA和1 mg/LBA的新鲜培养基上，可迅速分化成芽。 当带芽的愈伤组织也转移到无激素的培养基中时，少数根被诱导生长。到目前为止，在供试的10个品种中，有5个品种的未成熟叶片在离体条件下诱导出了芽
The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested