Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage
Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage
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DOI：
10.1016/j.bone.2011.06.006
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发表时间：
2011-10-01
期刊：
影响因子：
4.1
通讯作者：
Riancho, Jose A.
中科院分区：
文献类型：
--
作者：
Delgado-Calle, Jesus;Sanudo, Carolina;Riancho, Jose A.
表观遗传机制在基因表达的组织特异性调控中起重要作用。本研究分析了组织非特异性碱性磷酸酶（ALPL）基因表达与其近端区域CpG岛甲基化的关系。通过真实的时间RT-qPCR分析原代人成骨细胞（hOB）、成骨细胞系MG-63、乳腺细胞系MCF-7和骨组织中的基因表达。通过qMSP分析了这些细胞以及衬里成骨细胞和通过激光辅助捕获从人骨样品获得的骨细胞中的DNA甲基化。hOB表达的ALPL mRNA比MG-63细胞多得多（分别为7.3 +/-3.2对0.2 +/-0.1任意单位）。hOBs显示出非常弱的DNA甲基化（90%），而衬里成骨细胞显示出中等程度的甲基化（58 +/-13%）。这些结果表明DNA甲基化在成骨细胞-骨细胞转化过程中ALPL表达的调控中起重要作用。(C)2011 Elsevier Inc. All rights reserved.
Epigenetic mechanisms play an important role in the tissue-specific regulation of gene expression. This study analyzed the relationship between tissue non-specific alkaline phosphatase (ALPL) gene expression and the methylation of a CpG island located in its proximal region. Gene expression was analyzed by real time RT-qPCR in primary human osteoblasts (hOBs), the osteoblastic cell line MG-63, the mammary cell line MCF-7, and bone tissue. DNA methylation was analyzed by qMSP in those cells and also in lining osteoblasts and in osteocytes obtained from human bone samples by laser-assisted capture. hOBs expressed much more ALPL mRNA than MG-63 cells (7.3 +/- 3.2 vs. 0.2 +/- 0.1 arbitrary units, respectively). hOBs showed a very weak DNA methylation (90%), whereas lining osteoblasts showed an intermediate degree of methylation (58 +/- 13%). These results suggest an important role of DNA methylation in the regulation of ALPL expression through the osteoblast-osteocyte transition. (C) 2011 Elsevier Inc. All rights reserved.