The distribution and phylogenetic significance of a 50-kb chloroplast DNA inversion in the flowering plant family leguminosae
The distribution and phylogenetic significance of a 50-kb chloroplast DNA inversion in the flowering plant family leguminosae
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DOI：
10.1006/mpev.1996.0038
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发表时间：
1996-04-01
影响因子：
4.1
通讯作者：
Palmer, JD
中科院分区：
文献类型：
--
作者：
Doyle, JJ;Doyle, JL;Palmer, JD
与陆地植物中最常见的基因序列相比，豆科植物约650个属中的9个物种在其叶绿体基因组中具有较大的(50kb)倒位。据推测，该家族的基本成分尚未调查到倒位，这在豆科之外是未知的。利用聚合酶链式反应和限制性内切酶图谱相结合的方法，利用位于倒位终点两侧的引物或杂交探针，对132个豆科植物进行了倒位的筛选。在3个亚科中的2个亚科(含羞亚科和凯撒亚科)的所有分类群中均未发现倒位现象，而在第三亚科(凤蝶亚科)的大多数分类群中发现倒位现象，两个凤蝶亚族Swartzieae和Sophoreae的倒位现象是异质性的，这与许多证据表明这两个亚科的多植性是一致的。50kb的倒位似乎是豆科进化中的一个独特事件，为包括大多数凤蝶亚科的一个分支提供了一个同形异构体。(C)1996年学术出版社。
Species in 9 of the approximately 650 genera of the flowering plant family Leguminosae are known to possess a large (50-kb) inversion in their chloroplast genomes, relative to the gene order found most commonly among land plants. Putatively basal elements of the family have not been surveyed for the inversion, which is unknown outside the legumes. Using a combination of polymerase chain reaction and restriction-mapping approaches employing primers or hybridization probes flanking inversion endpoints, 132 legume genera were screened for the presence of the inversion. The inversion was found to be absent in all taxa from two of the three subfamilies (Mimosoideae and Caesalpinioideae), whereas the inversion was found to be present in most taxa of the third subfamily (Papilionoideae), Two papilionoid tribes, Swartzieae and Sophoreae, were heterogeneous for the inversion, which is consistent with a number of lines of evidence suggesting the polyphyly of these tribes. The 50-kb inversion appears to be a unique event in the evolution of Leguminosae, providing a synapomorphy for a clade that includes most of the Papilionoideae. (C) 1996 Academic Press, Inc.