Placental lipid droplet composition: Effect of a lifestyle intervention (UPBEAT) in obese pregnant women
Placental lipid droplet composition: Effect of a lifestyle intervention (UPBEAT) in obese pregnant women
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DOI：
10.1016/j.bbalip.2018.04.020
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发表时间：
2018-09-01
影响因子：
4.8
通讯作者：
Larque, Elvira
中科院分区：
文献类型：
--
作者：
Gazquez, Antonio;Uhl, Olaf;Larque, Elvira
母亲肥胖与不良结局相关。胎盘脂滴（LD）与母胎脂质转移有关，但尚不清楚胎盘LD脂肪成分是否可改变。我们评估了肥胖孕妇的饮食和体力活动干预与常规产前护理（UPBEAT研究）对胎盘LD组成的影响。LD通过超离心分离。通过LC-MS/MS分析总FA和磷脂（磷脂酰胆碱，PC;鞘磷脂，SM和溶血磷脂酰胆碱，Lyso-PC）。通过蛋白质印迹评估胎盘MFSD 2a表达。来自肥胖妇女的胎盘LD主要由饱和和单不饱和FA组成。干预组（n = 20）和对照组（n = 23）之间的TG和Chol组成相似。LD组中含有二高-γ-亚麻酸的PC与妊娠期体重增加呈正相关（P < 0.007），干预后降低。在整个样本中，携带DHA和花生四烯酸的PC与胎盘重量呈负相关。胎盘MFSD 2a表达与DHA脐带血代谢物相关，并且观察到LD脂质（尤其是携带DHA的物种）与脐带血代谢物之间的关系。我们首次描述了胎盘LD成分，并证明了肥胖孕妇生活方式干预对LD FAs的适度潜在有益作用。
Maternal obesity is associated with adverse outcomes. Placental lipid droplets (LD) have been implicated in maternal-fetal lipid transfer but it is not known whether placental LD fat composition is modifiable. We evaluated the effects of a diet and physical activity intervention in obese pregnant women compared to routine antenatal care (UPBEAT study) on placental LD composition. LD were isolated by ultracentrifugation. Total FAs and phospholipids (phosphatidylcholines, PCs; sphingomyelins, SMs and lyso-phosphatidylcholines, Lyso-PCs) were analyzed by LC-MS/MS. Placenta MFSD2a expression was assessed by western blot. Placental LDs from obese women were comprised of predominantly saturated and monounsaturated FAs. TG and Chol composition was similar between intervention (n = 20) and control (n = 23) groups. PCs containing dihomo-gamma-linolenic acid in LD were positively associated with gestational weight gain (P < 0.007), and lowered by the intervention. In the whole sample, PCs carrying DHA and arachidonic acid were inversely associated with placental weight. Placenta MFSD2a expression was associated with DHA cord blood metabolites and relationships were observed between LD lipids, especially DHA carrying species, and cord blood metabolites. We describe placenta LD composition for the first time and demonstrate modest, potentially beneficial effects of a lifestyle intervention on LD FAs in obese pregnant women.