Experimental Evidence Pointing to Rain as a Reservoir of Tomato Phyllosphere Microbiota
Experimental Evidence Pointing to Rain as a Reservoir of Tomato Phyllosphere Microbiota
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DOI：
10.1094/pbiomes-04-21-0025-r
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发表时间：
2021-01-01
影响因子：
4.4
通讯作者：
Vinatzer, Boris A.
中科院分区：
文献类型：
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作者：
Mechan-Llontop, Marco E.;Tian, Long;Vinatzer, Boris A.
植物微生物群在植物健康和作物生产力中起着至关重要的作用。群落组成的比较表明，种子，土壤和大气作为叶际微生物群的水库。在发现暴露于雨水的番茄（Solanum lycopersicum）植物的叶子比未暴露于雨水的番茄植物的叶子携带更高的微生物种群大小之后，我们通过实验测试了雨水是迄今为止被忽视的叶际微生物群的水库的假设。因此，将雨水微生物群与用浓缩雨水微生物群、过滤灭菌雨水或无菌水处理的番茄植物的叶圈微生物群进行比较。基于16S核糖体RNA扩增子测序，104个操作分类单位（OTU）在接种浓缩雨水微生物群后相对丰度显著增加，但在用无菌水或过滤灭菌的雨水处理后没有OTU显著增加。这104个OTU所属的属中的一些也在暴露于室外雨水的番茄植物上比在商业温室中生长的免受雨水影响的番茄植物上以更高的相对丰度被发现。总之，这些结果表明，降水作为叶圈微生物群的水库，并显示了受控实验的潜力，以调查不同水库的叶圈微生物群的组装中的作用。
Plant microbiota play essential roles in plant health and crop productivity. Comparisons of community composition have suggested seed, soil, and the atmosphere as reservoirs of phyllosphere microbiota. After finding that leaves of tomato (Solanum lycopersicum) plants exposed to rain carried a higher microbial population size than leaves of tomato plants not exposed to rain, we experimentally tested the hypothesis that rain is a thus-far-neglected reservoir of phyllosphere microbiota. Therefore, rain microbiota were compared with phyllosphere microbiota of tomato plants either treated with concentrated rain microbiota, filter-sterilized rain, or sterile water. Based on 16S ribosomal RNA amplicon sequencing, 104 operational taxonomic units (OTUs) significantly increased in relative abundance after inoculation with concentrated rain microbiota but no OTU significantly increased after treatment with either sterile water or filter-sterilized rain. Some of the genera to which these 104 OTUs belonged were also found at higher relative abundance on tomato plants exposed to rain outdoors than on tomato plants grown protected from rain in a commercial greenhouse. Taken together, these results point to precipitation as a reservoir of phyllosphere microbiota and show the potential of controlled experiments to investigate the role of different reservoirs in the assembly of phyllosphere microbiota.