Development of the Particle Inflow Gun
Development of the Particle Inflow Gun
复制标题
粒子流入枪的开发
DOI：
--
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发表时间：
1993
影响因子：
3
通讯作者：
J. Finer
中科院分区：
文献类型：
--
作者：
P. Vain;N. Keen;J. Murillo;C. Rathus;Cheri Nemes;J. Finer
设计了一种简单、廉价的粒子加速装置，用于将DNA直接输送到植物细胞中。粒子流入枪（PIG）是基于直接在氦蒸汽中加速DNA涂层的钨粒子。轰击玉米和大豆的胚性悬浮培养物以及豇豆的叶组织后，获得了高水平的β-葡萄糖醛酸酶基因的瞬时表达。利用该轰击装置还获得了大豆和玉米的稳定转化。
A simple and inexpensive particle acceleration apparatus was designed for direct delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles directly in a helium steam. High levels of transient expression of theβ-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of maize and soybean, and leaf tissue of cowpea. Stable transformation of soybean and maize has also been obtained using this bombardment apparatus.