Molecular and immunological dissection of diffuse large B cell lymphoma:: CD5+, and CD5- with CD10+ groups may constitute clinically relevant subtypes
Molecular and immunological dissection of diffuse large B cell lymphoma:: CD5+, and CD5- with CD10+ groups may constitute clinically relevant subtypes
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DOI：
10.1038/sj.leu.2401487
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发表时间：
1999-09-01
期刊：
影响因子：
11.4
通讯作者：
Seto, M
中科院分区：
文献类型：
--
作者：
Harada, S;Suzuki, R;Seto, M
弥漫性大B细胞淋巴瘤（DLBL）占成人非霍奇金淋巴瘤的最大比例，代表了一个多样化的淋巴肿瘤谱。对63例DLBL病例的临床病理、表型和基因型结果进行相关性分析和比较，以研究它们是否代表临床相关亚型。11例为I组（CD 5+），19例为II组（CD 5-CD 10+），33例为III组（CD 5-CD 10-）。通过观察BCL 2和BCL 6各自的基因重排和表达来关联数据。在临床方面，I组病例的生存率明显低于其他两组（log-rank检验，P = 0.016）。虽然BCL 2和BCL 6重排没有表现出任何倾向于一个特定的亚组，BCL 2的免疫组化检测是不太频繁，在统计学显著水平（P=0.011），在组II（50%）比组I（82%）和III（82%）的情况下。这似乎证实了CD 5（-）CD 10（+）型DLBL的独特方面，表明与通常缺乏BCL 2表达的正常生发中心细胞有一定的关系。BCL-6蛋白表达检测在大多数目前DLBL的情况下（92%），无论这一分组。这些数据表明，通过检测CD 5和CD 10的表型划分将提高我们对DLBL的理解，并有助于未来DLBL的亚组。
Diffuse large B cell lymphoma (DLBL) constitutes the greatest percentage of adult non-Hodgkin's lymphomas and represents a diverse spectrum of lymphoid neoplasms. Clinicopathologic, phenotypic and genotypic findings were correlated and compared for 63 DLBL cases to investigate whether they represent clinically relevant subtypes. They were all cyclin D1 negative and were phenotypically divided into three groups, ie group I (CD5(+) type, n=11), group Il (CD5(-) CD10(+) type, n=19), and group III (CD5(-) CD10(-) type, n=33). Data were correlated by observing the respective gene rearrangement and expression of BCL2 and BCL6. In clinical aspects, the group I cases demonstrated a significantly inferior survival than those of the other two groups (log-rank test, P = 0.016). Although rearrangement of BCL2 and BCL6 did not show any inclination to a specific subgroup, the immunohistochemical detection of BCL2 was less frequent, at a statistically significant level(P=0.011), in group Il (50%) than in group I (82%) and III(82%) cases. This appears to confirm the unique aspect of the CD5(-) CD10(+) type DLBL, indicating a certain relationship with the normal germinal center cells which usually lack BCL2 expression. The BCL6 protein expression was detected in most of the present DLBL cases (92%) irrespective of this grouping. These data suggest that the phenotypic delineation by the detection of CD5 and CD10 will improve our understanding of DLBL and be helpful in a future subgrouping of DLBL.