Standardized method for solubility and storage of capsaicin-based solutions for cough induction.
Standardized method for solubility and storage of capsaicin-based solutions for cough induction.
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DOI：
10.1186/1745-9974-10-6
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Davenport PW
中科院分区：
文献类型：
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作者：
Costanzo MT;Yost RA;Davenport PW
不同实验室制备用于诱导咳嗽的吸入辣椒素溶液的方法差异很大，这导致致咳激发试验结果之间存在不一致。在这些辣椒素溶液中添加吐温可提高溶解度和稳定性；然而，吐温的恶臭味道使得长时间吸入该溶液会感到不愉快。我们试图创建一种标准方法，在不添加吐温的情况下制备可溶且稳定的辣椒素溶液（10% 乙醇/水）。在各种溶剂系统中（含或不含吐温）制备了浓度范围为 0 至 500 μM 的辣椒素溶液。将样品储存在四种不同的环境中（-20°C、3°C 和室温，避光；以及室温，暴露在光线下）以测试稳定性。通过紫外吸收法检测辣椒素。对所有数据进行格鲁布检验以消除统计异常值。使用或不使用吐温制备的溶液中的辣椒素浓度相似（吐温使溶解度略有增加），但两种溶剂系统均未提供完全溶解度。在测试的四种环境中，在 3°C 避光条件下储存辣椒素溶液可提供最大的稳定性，至少可保存 30 周。我们建议使用不含吐温的 10% 乙醇/水溶剂系统来制备针对致咳挑战的辣椒素溶液。虽然该溶剂系统不能提供完全的溶解度，但我们详细介绍了一种辣椒素溶液的制备方法，该方法将解决这种溶解度的损失，同时保持溶液不含吐温且可供人类吸入安全。
Preparation of inhaled capsaicin solutions for cough induction varies greatly from one lab to another, which creates inconsistencies between tussigenic challenge results. The addition of Tween to these capsaicin solutions provides increased solubility and stability; however, the foul taste of Tween makes inhaling the solution for any prolonged period of time unpleasant. We sought to create a standard method for preparing soluble and stable capsaicin-based solutions (in 10% ethanol/water), without the addition of Tween. Capsaicin solutions were created at concentrations ranging from 0 to 500 μM in a variety of solvent systems, with and without Tween. Samples were stored in four different environments (-20°C, 3°C, and room temperature, protected from light; and room temperature, exposed to light) to test stability. Detection of capsaicin was carried out by UV absorption. A Grubb’s test was performed on all data to remove statistical outliers. Similar capsaicin concentrations were seen for solutions prepared with or without Tween (Tween provided a slight increase in solubility), with neither solvent system providing complete solubility. Of the four environments tested, storing capsaicin solutions at 3°C while protected from light afforded the greatest stability, for a minimum of 30 weeks. We recommend the use of a 10% ethanol/water solvent system without Tween in the preparation of capsaicin solutions for tussigenic challenges. While this solvent system does not provide complete solubility, we have detailed a method for capsaicin solution preparation that will account for this loss of solubility, while maintaining a solution that is Tween-free and safe for human inhalation.