Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system
Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system
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DOI：
10.1038/nbt.2925
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发表时间：
2014-08-01
影响因子：
46.9
通讯作者：
Lopez-Rubio, Jose-Juan
中科院分区：
文献类型：
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作者：
Ghorbal, Mehdi;Gorman, Molly;Lopez-Rubio, Jose-Juan
疟原虫恶性疟原虫的基因组操作在很大程度上仍然难以处理，需要改进的基因组工具来进一步了解发病机制和耐药性。我们通过破坏染色体位点并高效地产生无标记的单核苷酸替换，展示了CRISPR - Cas9系统在恶性疟原虫中的应用。此外，通过引入一种先前涉及的多态性产生了一种青蒿素抗性菌株，从而说明了高效基因组编辑在疟疾研究中的价值。
Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.