Real-time monitoring of skin ethanol gas by a high-sensitivity gas phase biosensor (bio-sniffer) for the non-invasive evaluation of volatile compounds
Real-time monitoring of skin ethanol gas by a high-sensitivity gas phase biosensor (bio-sniffer) for the non-invasive evaluation of volatile compounds
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DOI：
10.1016/j.bios.2018.09.070
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发表时间：
2019-03-15
影响因子：
12.6
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
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作者：
Arakawa, Takahiro;Suzuki, Takuma;Mitsubayashi, Kohji
本研究构建了一种高灵敏度、高选择性的生化气体传感器（生物嗅探器）和皮肤气室实时监测系统，用于人体皮肤上乙醇气体浓度的检测。这种生物嗅探器根据烟酰胺腺嘌呤二核苷酸（NADH）的荧光强度的变化来测量乙醇的浓度，所述烟酰胺腺嘌呤二核苷酸（NADH）在醇脱氢酶（ADH）的酶促反应中产生。NADH检测系统采用紫外发光二极管（UV-LED）作为激发光，高灵敏度的光电倍增管作为荧光强度检测器。乙醇生物嗅探器的校准范围经验证为25 ppb至128 ppm。为了测量皮肤气体中的乙醇浓度，受试者摄入酒精饮料，并监测传感器输出。我们选择手掌的中心部分、手背和手腕作为目标。饮酒后，测量每个目标处的皮肤乙醇气体的实时浓度。饮水后70 min左右达到最大输出值，然后逐渐下降。我们发现，乙醇释放动力学是不同的，这取决于与开发的监测系统测量的手的一部分。因此，这种具有皮肤气室的高灵敏度和选择性生物嗅探器可用于测量皮肤表面上的乙醇，并可应用于呼吸和皮肤气体研究，以及用作临床诊断生物标志物的挥发性血液化合物的研究。
In this study, a highly sensitive and selective biochemical gas sensor (bio-sniffer) and real-time monitoring system with skin gas cell was constructed for the determination of ethanol gas concentration on human skin. This bio-sniffer measured the concentration of ethanol according to the change in fluorescence intensity of nicotinamide adenine dinucleotide (NADH), which is produced in an enzymatic reaction by alcohol dehydrogenase (ADH). The NADH detection system used an ultraviolet light emitting diode (UV-LED) as the excitation light, and a highly sensitive photomultiplier tube as a fluorescence intensity detector. The calibration range of the ethanol bio-sniffer was validated from 25 ppb to 128 ppm. To measure the concentration of ethanol within skin gas, subjects ingested an alcohol beverage, and the sensor output was monitored. We chose the central part of the palm, a back of the hand, and a wrist as targets. The real-time concentration of skin ethanol gas at each target was measured after drinking. The maximum output values were reached at approximately 70 min after drinking and then gradually decreased. We showed that ethanol release kinetics were different depending on the part of the hand measured with the developed monitoring system. Accordingly, this highly sensitive and selective biosniffer with a skin gas cell could be used to measure ethanol on the skin surface and could be applied for breath and skin gas research, as well as investigation of volatile blood compounds used as biomarkers for clinical diagnosis.