Secretion of the vasoactive peptides, endothelin, and parathyroid hormone-related peptide, by decidual explants from pregnancies complicated by intrauterine growth restriction.
Secretion of the vasoactive peptides, endothelin, and parathyroid hormone-related peptide, by decidual explants from pregnancies complicated by intrauterine growth restriction.
复制标题
妊娠时蜕膜外植体分泌血管活性肽、内皮素和甲状旁腺激素相关肽，并发宫内生长受限。
DOI：
10.1016/s1071-5576(99)00033-7
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Benoit,LA
中科院分区：
文献类型：
--
作者：
Heffner,LJ;Kumari,M;Benoit,LA
目的：探讨合并宫内生长受限（IUGR）妊娠蜕膜中内皮素和甲状旁腺激素相关肽（PTHrP）两种血管活性肽的体外分泌是否与正常妊娠有差异。方法：10例IUGR和9例胎龄匹配的对照妊娠，在分娩时收集蜕膜，体外培养24h。测定条件培养基中内皮素和PTHrP的浓度。乳酸脱氢酶在4和24小时释放到培养基中，在单独的一组中，4个IUGR和3个对照蜕膜。结果：IUGR妊娠组蜕膜内皮素和PTHrP分泌明显低于对照组（内皮素51±11比105±16 pg/100 mg组织/24 h， P = 0.01； PTHrP 164±17比641±209 ng/100 mg组织/24 h， P = 0.02）。乳酸脱氢酶在IUGR和对照培养基中的释放量无显著差异。结论：基于已知的内皮素和PTHrP对血管平滑肌的作用，最初预计IUGR蜕膜比对照组分泌更多的内皮素和更少的PTHrP。这两种多肽的减少不能用传递途径或整体组织功能障碍来解释。为了确定为什么这些激素在合并IUGR的妊娠中都降低，有必要对蜕膜外激素和PTHrP的潜在介质进行调查。
Objective:To determine whether in vitro secretion of two vasoactive peptides, endothelin and parathyroid hormone-related peptide (PTHrP), is different in decidua from pregnancies complicated by intrauterine growth restriction (IUGR) than in normal pregnancies.Methods:In ten IUGR and nine gestational age-matched control pregnancies, decidua was collected at delivery and explants cultured for 24 hours. Endothelin and PTHrP concentrations in the conditioned medium were determined. Lactate dehydrogenase release into the medium was measure at 4 and 24 hours in a separate group of four IUGR and three control decidua.Results:Endothelin and PTHrP secretion by the decidua of the IUGR pregnancies were markedly lower than those in controls (endothelin 51 ± 11 versus 105 ± 16 pg/100 mg tissue/24 hours, P = .01; PTHrP 164 ± 17 versus 641 ± 209 ng/100 mg tissue/24 hours, P = .02). Lactate dehydrogenase release into the medium was not different between the IUGR and control dedicua.Conclusions:Based on the known effects of endothelin and PTHrP on vascular smooth muscle, it was originally anticipated that more endothelin and less PTHrP would be secreted by the IUGR decidua than the control. The finding that both peptides decreased could not be explained by route of delivery or global tissue dysfunction. Investigation of potential mediators of decidual ET and PTHrP will be necessary to determine why these hormones are both decreased in pregnancies complicated by IUGR.