An in situ hybridization study of syndecan family during the late stages of developing mouse molar tooth germ
An in situ hybridization study of syndecan family during the late stages of developing mouse molar tooth germ
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DOI：
10.1007/s12565-022-00647-w
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发表时间：
2022-02-04
影响因子：
1.2
通讯作者：
Shibata，Shunichi
中科院分区：
文献类型：
--
作者：
Fujikawa，Kaoru;Nonaka，Naoko;Shibata，Shunichi
使用[35S]-UTP标记的cRNA探针，通过原位杂交研究了牙胚形成后期syndecan-1、2、3和4 mRNA的表达。在E18.5-P3.0期间，Syndecan-1mRNA主要表达于星状网和中间层以及牙乳头/牙囊的颈部区域。实时 RT-PCR 分析支持了出生后牙上皮表达的增强。这些时空表达模式可能表明syndecan-1在牙齿形成（如牙齿萌出或牙根形成）中的特定作用。Syndecan-3mRNA表达在E18.5时在成牙本质细胞中变得明显，但与在此阶段强烈表达的胶原型ImRNA相比，syndecan-3在成牙本质细胞中的表达在出生后仅限于牙尖下方的成熟成牙本质细胞中。实时RT-PCR分析也支持了这一结果，表明syndecan-3可能参与牙本质发生的进展而不是起始。Syndecan-4mRNA大致表现出与syndecan-3相似的表达模式。Syndecan-2mRNA在实验期间没有表现出显着表达，但实时RT-PCR分析表明syndecan-2表达可能随着硬组织形成而增强。
Expression ofsyndecan-1, 2, 3, and4mRNAs during the late stages of tooth germ formation was investigated by in situ hybridization, using [35S]-UTP-labeled cRNA probes.Syndecan-1mRNA was mainly expressed in the stellate reticulum and stratum intermedium as well as at the cervical region of dental papilla/dental follicle during E18.5-P3.0. Expression in the dental epithelium was enhanced during the postnatal periods, which was supported by real-time RT-PCR analysis. These spatiotemporal expression patterns may suggest specific roles of syndecan-1 in tooth formation such as tooth eruption or root formation.Syndecan-3mRNA expression became evident in odontoblasts at E18.5, but compared tocollagen type ImRNA, which was strongly expressed at this stage,syndecan-3expression in odontoblast was restricted in mature odontoblasts beneath the cusps during the postnatal periods. This result was also supported by real-time RT-PCR analysis, and indicated that syndecan-3 may be involved in the progress of dentinogenesis rather than in the initiation of it.Syndecan-4mRNA roughly showed comparable expression patterns to those ofsyndecan-3.Syndecan-2mRNA did not show significant expression during the experimental period, but real-time RT-PCR analysis suggested thatsyndecan-2expression might be enhanced with hard tissue formation.