Pneumolysin plays a key role at the initial step of establishing pneumococcal nasal colonization
Pneumolysin plays a key role at the initial step of establishing pneumococcal nasal colonization
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DOI：
10.1007/s12223-016-0445-z
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发表时间：
2016-09-01
影响因子：
2.6
通讯作者：
Yamanaka, Noboru
中科院分区：
文献类型：
--
作者：
Hotomi, Muneki;Yuasa, Jun;Yamanaka, Noboru
肺炎链球菌在鼻咽部的定植是肺炎链球菌感染后续发展的重要初始步骤。肺炎球菌有许多毒力因子，它们在定植过程中发挥作用。肺炎溶血素(PLY)是肺炎球菌侵袭性疾病的关键毒力因子，可导致严重的组织损伤和炎症，并破坏上皮紧密连接。在这项研究中，我们使用小鼠定植模型评估了PLY在肺炎链球菌鼻腔定植中的作用。在鼻腔攻击后的第1天和第2天，观察到从鼻腔组织中恢复的PLY缺陷肺炎球菌的数量以及鼻腔冲洗的数量减少，但不是在以后。这一发现有力地支持了PLY在最初建立鼻部定植中的重要作用。依赖PLY的局部鼻黏膜侵袭可能需要肺炎链球菌的鼻腔定植。这些数据有助于解释为什么以无症状殖民者的身份存在的有机体进化出毒力因子，使其能够偶尔入侵并杀死宿主。因此，同样的肺炎球菌毒力因子，可以杀死宿主的PLE，也可能在鼻咽携带的建立早期发挥作用。
Nasopharyngeal colonization by Streptococcus pneumoniae is an important initial step for the subsequent development of pneumococcal infections. Pneumococci have many virulence factors that play a role in colonization. Pneumolysin (PLY), a pivotal pneumococcal virulence factor for invasive disease, causes severe tissue damage and inflammation with disruption of epithelial tight junctions. In this study, we evaluated the role of PLY in nasal colonization of S. pneumoniae using a mouse colonization model. A reduction of numbers of PLY-deficient pneumococci recovered from nasal tissue, as well as nasal wash, was observed at days 1 and 2 post-intranasal challenges, but not later. The findings strongly support an important role for PLY in the initial establishment nasal colonization. PLY-dependent invasion of local nasal mucosa may be required to establish nasal colonization with S. pneumoniae. The data help provide a rationale to explain why an organism that exists as an asymptomatic colonizer has evolved virulence factors that enable it to occasionally invade and kill its hosts. Thus, the same pneumococcal virulence factor, PLY that can contribute to killing the host, may also play a role early in the establishment of nasopharynx carriage.