Multispectral singlet oxygen luminescent dosimetry (MSOLD) for Photofrin-mediated Photodynamic Therapy.
Multispectral singlet oxygen luminescent dosimetry (MSOLD) for Photofrin-mediated Photodynamic Therapy.
复制标题
用于 Photofrin 介导的光动力疗法的多光谱单线态氧发光剂量测定 (MSOLD)。
DOI：
10.1117/12.2652590
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Zhu,TimothyC
中科院分区：
文献类型：
--
作者：
Yang,Weibing;Rastogi,Vivek;Sun,Hongjing;Sharma,Dvij;Wilson,BrianC;Hadfield,RobertH;Zhu,TimothyC
直接检测单重态氧（[1O2]）是II型PDT的圣杯剂量学方法，这一目标可以使用多光谱单重态氧剂量法（MSOLD）进行量化。然而，产生的单线态氧信号的寿命短和极弱的性质导致需要提高MSOLD的信噪比。本研究探讨了优化MSOLD信号采集的方法，特别是采用检测光和PDT处理光之间的正交排列，由两个光纤组成-分别连接到632 nm激光器和InGaAs探测器。然后，InGaAs探测器收集的光通过滤光轮，在1200 nm、1240 nm、1250 nm、1270 nm和1300 nm处进行光谱发射测量。对荧光背景进行拟合，并对单线态氧峰进行高斯拟合，建立了减去背景的单线态氧发射信号的数据。然后将MSOLD信号与单线态氧显剂量法（SOED）结果进行比较，SOED基于直接测量体内光通量（速率）、体内光蛋白浓度和组织氧合浓度。本研究的重点是验证MSOLD信号在各种体外条件下的灵敏度和最小可检测性。最后，MSOLD装置将在光晶体介导的放射诱导纤维肉瘤（RIF）肿瘤小鼠PDT中进行测试。
Direct detection of singlet-state oxygen ([1O2]) constitutes the holy grail dosimetric method for type II PDT, a goal that can be quantified using multispectral singlet oxygen dosimetry (MSOLD). However, the short lifetime and extremely weak nature of the singlet oxygen signal produced has given rise to a need to improve MSOLD signal-to-noise ratio. This study examines methods for optimizing MSOLD signal acquisition, specifically employing an orthogonal arrangement between detection and PDT treatment light, consisting of two fiber optics - connected to a 632-nm laser and an InGaAs detector respectively. Light collected by the InGaAs detector is then passed through a filter wheel, where spectral emission measurements are taken at 1200 nm, 1240 nm, 1250 nm, 1270 nm, and 1300 nm. The data, after fitting to the fluorescence background and a gaussian-fit for the singlet oxygen peak, is established for the background-subtracted singlet oxygen emission signal. The MSOLD signal is then compared with the singlet oxygen explicit dosimetry (SOED) results, based on direct measurements of in-vivo light fluence (rate), in-vivo Photofrin concentration, and tissue oxygenation concentration. This study focuses on validating the sensitivity and minimum detectability of MSOLD signal in various in-vitro conditions. Finally, the MSOLD device will be tested in Photofrin-mediated PDT for mice bearing Radiation- Induced Fibrosarcoma (RIF) tumors.