Phylogenetic analysis of Bacillus cereus isolates from severe systemic infections using multilocus sequence typing scheme
Phylogenetic analysis of Bacillus cereus isolates from severe systemic infections using multilocus sequence typing scheme
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DOI：
10.1111/j.1348-0421.2006.tb03847.x
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Ohta, Michio
中科院分区：
文献类型：
--
作者：
Vassileva, Maria;Torii, Keizo;Ohta, Michio
应用蜡状芽孢杆菌MLST数据库中的多位点序列分型方案，对包括呼吸道症状在内的严重或致死性全身感染病例中的蜡状芽孢杆菌菌株进行分析。这些分离物均匀分布在两个主要分支之间，其中60%的分离物具有数据库中新的等位基因图谱。收集的菌株中有一半聚集在与炭疽芽孢杆菌系统发育起源相邻的谱系中。来自有呼吸道症状的致命病例的菌株被分配到两个主要分支。这是引起呼吸道症状的菌株被鉴定为与炭疽杆菌遗传距离较远的第一份报告。将这里提供的菌株的系统发育位置与之前提交给数据库的所有系统性感染分离株进行比较，发现它们出现在来自其他研究的临床分离株所在的同一簇中。致病菌株似乎在系统发育树上形成了簇。
Bacillus cereus strains from cases of severe or lethal systemic infections, including respiratory symptoms cases, were analyzed using multilocus sequence typing scheme of B. cereus MLST database. The isolates were evenly distributed between the two main clades, and 60% of them had allele profiles new to the database. Half of the collection's strains clustered in a lineage neighboring Bacillus anthracis phylogenetic origin. Strains from lethal cases with respiratory symptoms were allocated in both main clades. This is the first report of strains causing respiratory symptoms to be identified as genetically distant from B. anthracis. The phylogenetic location of the presented here strains was compared with all previously submitted to the database isolates from systemic infections, and were found to appear in the same clusters where clinical isolates from other studies had been assigned. It seems that the pathogenic strains are forming clusters on the phylogenetic tree.