Sample stacking in capillary zone electrophoresis: Principles, advantages and limitations
Sample stacking in capillary zone electrophoresis: Principles, advantages and limitations
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DOI：
10.1002/1522-2683(20000801)21:14
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发表时间：
2000-08
期刊：
影响因子：
2.9
通讯作者：
J. Beckers;P. Boček
中科院分区：
文献类型：
--
作者：
J. Beckers;P. Boček
描述了堆积过程的原理，并讨论了它们的性质，包括区域边界行为的基本原理以及移动边界电泳、等速电泳法和区域电泳中边界自校正的结果。此外，还描述了堆积过程的各种可能性和不可避免的去堆积，并给出了几个实际应用的堆积过程的例子，此外还有许多关于应用的参考。讨论了堆积过程中使用的一些限制。这篇论文的编排方式既可以作为该领域的介绍，也可以作为参考概述。
The principles of stacking procedures are described and their properties are discussed, including the fundamentals of the behavior of zone boundaries and the consequences of the self‐correcting properties of boundaries in moving boundary electrophoresis, isotachophoresis, and zone electrophoresis. Further, the diverse possibilities of stacking procedures and the unavoidable destacking are described, and several examples of practically applied stacking procedures are given, besides many references to applications. Some limitations in the use of stacking procedures are discussed. The paper is arranged in such a way that it can serve both as an introduction into the field and as a reference overview.