Ectopic bone formation in bone marrow stem cell seeded calcium phosphate scaffolds as compared to autograft and (cell seeded) allograft
Ectopic bone formation in bone marrow stem cell seeded calcium phosphate scaffolds as compared to autograft and (cell seeded) allograft
复制标题
DOI：
10.22203/ecm.v014a03
复制
发表时间：
2007-07-01
影响因子：
3.1
通讯作者：
de Bruijn, J. D.
中科院分区：
文献类型：
--
作者：
Eniwumide, J. O.;Yuan, H.;de Bruijn, J. D.
需要改进目前的治疗策略来治疗骨骼缺陷。骨组织工程为这些策略提供了潜在的优势。在这项研究中，评估了一系列支架中的异位骨形成。活体自体移植物和失活的同种异体移植物作为对照，实验组分别包括自体骨髓来源的干细胞接种的同种异体移植物、双相磷酸钙（BCP）和磷酸三钙（TCP）。所有植入物均植入成年荷兰奶山羊的背部肌肉中12周。进行微计算机断层扫描（mu CT）分析和组织形态计量学，以评估和量化异位骨形成。在良好的一致性，无论是mu CT和组织形态学分析表明，骨形成的细胞接种的磷酸钙支架相比，自体移植物，同种异体移植物和细胞接种的同种异体移植物植入物显着增加。通过组织学观察到自体移植物、同种异体移植物和细胞接种同种异体移植物植入物的广泛吸收，并通过组织形态计量学证实。细胞接种的TCP植入物也显示出明显的组织形态学和μ CT降解迹象，而细胞接种的BCP植入物的降解可以忽略不计。这些结果表明，细胞接种的磷酸钙支架在异位植入部位的骨形成方面上级自体移植物、同种异体移植物或细胞接种的同种异体移植物。此外，证明了mu CT用于矿化骨和磷酸钙支架的有效和非破坏性分析的有用性。
Improvements to current therapeutic strategies are needed for the treatment of skeletal defects. Bone tissue engineering offers potential advantages to these strategies. In this study, ectopic bone formation in a range of scaffolds was assessed. Vital autograft and devitalised allograft served as controls and the experimental groups comprised autologous bone marrow derived stem cell seeded allograft, biphasic calcium phosphate (BCP) and tricalcium phosphate (TCP), respectively. All implants were implanted in the back muscle of adult Dutch milk goats for 12 weeks. Micro-computed tomography (mu CT) analysis and histomorphometry was performed to evaluate and quantify ectopic bone formation. In good agreement, both mu CT and histomorphometric analysis demonstrated a significant increase in bone formation by cell-seeded calcium phosphate scaffolds as compared to the autograft, allograft and cell-seeded allograft implants. An extensive resorption of the autograft, allograft and cell-seeded allograft implants was observed by histology and confirmed by histomorphometry. Cell-seeded TCP implants also showed distinct signs of degradation with histomorphometry and mu CT, while the degradation of the cell-seeded BCP implants was negligible. These results indicate that cell-seeded calcium phosphate scaffolds are superior to autograft, allograft or cell-seeded allograft in terms of bone formation at ectopic implantation sites. In addition, the usefulness of mu CT for the efficient and non-destructive analysis of mineralised bone and calcium phosphate scaffold was demonstrated.