Antioxidative catechol lignans/neolignans isolated from defatted kernel of Jatropha curcas
Antioxidative catechol lignans/neolignans isolated from defatted kernel of Jatropha curcas
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DOI：
10.1007/s10086-016-1563-6
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发表时间：
2016-08-01
影响因子：
2.9
通讯作者：
Pankasemsuk, Tanachai
中科院分区：
文献类型：
--
作者：
Suzuki, Toshisada;Eto, Kyoko;Pankasemsuk, Tanachai
麻疯树在许多国家用于生产生物柴油，在生产过程中会产生大量的脱脂果仁作为副产品。研究了麻疯树仁脱脂后的酚类物质含量和抗氧化活性。用Folin-Ciocalteu法和DPPH(2，2-二苯基-1-苦基肼)法分别测定了乙酸乙酯有机提取物、乙酸乙酯水提取物、甲醇有机提取物和甲醇水提取物的酚类物质含量和抗氧化活性。甲醇有机组分中酚类化合物含量较高，具有较强的抗氧化活性。从甲醇有机组分中分离得到8个化合物，鉴定为儿茶酚型木脂素(3，3‘-二二甲基松脂醇)、5个新木脂素(异美洲油醇A、9’-O-甲基异美洲油醇A、9‘-O-甲基美洲油醇A、9’-O-甲基美洲油醇A和异美洲油素A)和2个倍半木脂素(异丙肾上腺素、普林西平)。含两个邻苯二酚基团的3，3‘-二去甲基松脂酚(IC50：16.0 mU ie)、异丙肾上腺素(9.12 mU ie)和大黄素(11.7 mie)的抗氧化活性高于特罗克斯(21.5 mU ie)。异美洲醇A对生物柴油的氧化有抑制作用。这些结果表明，麻疯树脱脂后的果仁是天然抗氧化剂的来源，可用于麻疯树生物柴油工业的稳定剂。
Jatropha curcas L. has been cultivated in many countries for biodiesel production, and defatted kernel is generated in huge quantities as a by-product in the production. This study investigated the phenolic content and antioxidative activity of the defatted kernel of Jatropha. Four extracts, EtOAc-organic, EtOAc-water, MeOH-organic, and MeOH-water fractions, were tested for phenolic content and antioxidative activity using the Folin-Ciocalteu method and the DPPH (2, 2-diphenyl-1-picrylhydrazyl) method, respectively. The MeOH-organic fraction showed a high content of phenolic compounds and exhibited a relatively strong antioxidative activity. Eight compounds were isolated from the MeOH-organic fraction, and identified as a catechol-type lignan (3,3'- bisdemethylpinoresinol), five neolignans (isoamericanol A, americanol A, 9'-O-methylisoamericanol A, 9'-O-methylamericanol A, and isoamericanin A), and two sesquineolignans (isoprincepin, princepin). The antioxidative activities of 3,3'-bisdemethylpinoresinol (IC50: 16.0 mu Ie), isoprincepin (9.12 mu Ie), and princepin (11.7 mu Ie), which have two catechol groups were higher than that of Trolox (21.5 mu Ie). The isoamericanol A inhibited the oxidation of biodiesel. These results indicate that the defatted kernel of Jatropha is a source of natural antioxidants that would applicable for stabilizers in Jatropha biodiesel industries.