Skin and plasma carotenoid response to a provided intervention diet high in vegetables and fruit: uptake and depletion kinetics
Skin and plasma carotenoid response to a provided intervention diet high in vegetables and fruit: uptake and depletion kinetics
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DOI：
10.3945/ajcn.114.086900
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发表时间：
2014-09-01
影响因子：
7.1
通讯作者：
Whigham, Leah D.
中科院分区：
文献类型：
--
作者：
Jahns, Lisa;Johnson, Luann K.;Whigham, Leah D.
背景：目的需要生物标志物来评估蔬菜和水果干预试验的依从性。血液类胡萝卜素被认为是蔬菜和水果摄入量的最佳生物标志物，但采集血液是侵入性的，而且对人口研究进行分析相对昂贵。共振拉曼光谱（RRS）是一种无创评估皮肤类胡萝卜素的创新方法。目的：我们的目的是比较血液类胡萝卜素浓度和皮肤类胡萝卜素的RRS评估在控制喂养干预期间。设计：29名参与者食用低类胡萝卜素饮食（6周，第1和第3阶段），提供含有6杯等量（1046克/天）蔬菜和水果的饮食（8周，第2阶段）和常规饮食（最后8周，第4阶段）。结果：基线时，皮肤和血浆总类胡萝卜素值相关（r = 0.61, P < 0.001）。第1期结束时，皮肤和血浆类胡萝卜素值分别下降了36%和30% (P < 0.001)，第2期结束时，类胡萝卜素值上升了50% （P < 0.001）。血浆类胡萝卜素在第三阶段中期恢复到基线浓度，皮肤类胡萝卜素在第四阶段中期恢复到基线浓度。皮肤类胡萝卜素状态通过使用包括所有时间点的混合线性模型预测血浆值（r = 0.72, P < 0.001），这表明皮肤类胡萝卜素状态的变化与血浆在大范围摄入中的变化密切相关。在个体水平上，皮肤类胡萝卜素在所有时间点上预测血浆值（r = 0.70, P < 0.001）。结论：用共振拉曼光谱评估皮肤类胡萝卜素状态是一种无创、客观的蔬菜和水果摄入量变化的生物标志物。该试验在clinicaltrials.gov注册为NCT01403844。
Background: Objective biomarkers are needed to assess adherence to vegetable and fruit intervention trials. Blood carotenoids are considered the best biomarker of vegetable and fruit intake, but collecting blood is invasive and the analyses are relatively expensive for population studies. Resonance Raman spectroscopy (RRS) is an innovative method for assessing carotenoids in skin noninvasively.Objective: Our objective was to compare blood carotenoid concentrations with skin carotenoid assessments by RRS during a controlled feeding intervention.Design: Twenty-nine participants consumed low-carotenoid diets (6 wk, phases 1 and 3), a provided diet containing 6-cup equivalents (1046 g/d) of vegetables and fruit (8 wk, phase 2), and usual diet (final 8 wk, phase 4).Results: At baseline, skin and plasma total carotenoid values were correlated (r = 0.61, P < 0.001). Skin and plasma carotenoid values decreased (P < 0.001) 36% and 30%, respectively, from baseline to the end of phase 1 and then increased (P < 0.001) by >200% at the end of phase 2. Plasma carotenoids returned to baseline concentrations by the middle of phase 3 and skin carotenoid concentrations by the middle of phase 4. Skin carotenoid status predicted plasma values by using a mixed linear model including all time points (r = 0.72, P < 0.001), which indicates that changes in skin carotenoid status closely follow changes in plasma across a broad range of intakes. At the individual level, skin carotenoids predicted plasma values (r = 0.70, P < 0.001) over all time points.Conclusion: Skin carotenoid status assessed by resonance Raman spectroscopy is a noninvasive, objective biomarker of changes in vegetable and fruit intake. This trial was registered at clinicaltrials.gov as NCT01403844.