Transgenic mice carrying yeast artificial chromosomes.
Transgenic mice carrying yeast artificial chromosomes.
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DOI：
10.1017/s1462399403006082
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发表时间：
2003-04-07
影响因子：
6.2
通讯作者：
Peterson, Kenneth R
中科院分区：
文献类型：
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作者：
Peterson, Kenneth R
酵母人工染色体（YAC）转基因小鼠的产生已被证明是一种有价值的系统：（1）研究基因结构-功能关系;（2）产生人类疾病的小鼠模型;（3）补充小鼠突变体;（4）产生小鼠生物反应器;和（5）筛选体内YAC文库。当前技术的不断改进和新协议的开发应鼓励广泛适应这些和其他应用的这一战略。使用整个基因座作为转基因是鼠转基因的重要改进，因为它导致个体发育期间更真实的基因表达模式和水平。应用这项技术开发人类人工染色体（HACs）可能会提供下一代基因治疗载体，克服与当前载体系统相关的大多数问题和障碍。
The generation of transgenic mice with yeast artificial chromosomes (YACs) has proven to be a valuable system to: (1) study gene structure-function relationships; (2) produce mouse models of human disease; (3) complement mouse mutants; (4) generate mice bioreactors; and (5) screen YAC libraries in vivo. Continued refinement of current techniques and development of new protocols should encourage widespread adaptation of this strategy for these and other applications. Use of whole loci as transgenes is an important improvement in murine transgenesis because it results in a more realistic pattern and level of gene expression during ontogeny. Application of this technology to develop human artificial chromosomes (HACs) might provide the next generation of gene therapy vectors that will overcome most of the problems and barriers associated with current vector systems.