Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.
Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.
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DOI：
10.1016/j.abb.2013.06.016
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发表时间：
2013-09-01
影响因子：
3.9
通讯作者：
Hoffman, David W.
中科院分区：
文献类型：
--
作者：
Terrell, Cassidy R.;Burks, Elizabeth A.;Whitman, Christian P.;Hoffman, David W.
Methylibium petroleiphilumstrain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order to understand the roles of the pathways, the 4-oxalocrotonate tautomerase (4-OT) isozyme from each pathway was characterized. Tautomerase I and tautomerase II have the lowest pairwise sequence identity (35%) among the isozyme pairs in the parallel pathways, and could offer insight into substrate preferences and pathway functions. The kinetic parameters of tautomerase I and tautomerase II were determined using 2-hydroxymuconate and 5-(methyl)-2-hydroxymuconate. Both tautomerase I and tautomerase II process the substrates, but with different efficiencies. Crystal structures were determined for both tautomerase I and tautomerase II, at 1.57 and 1.64 Å resolution, respectively. The backbones of tautomerase I and tautomerase II are highly similar, but have distinct active site environments. The results, in combination with those for other structurally and kinetically characterized 4-OT isozymes, suggest that tautomerase I catalyzes the tautomerization of both 2-hydroxymuconate and alkyl derivatives, whereas tautomerase II might specialize in other aromatic hydrocarbon metabolites.
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影响因子：
2.9
作者：
Burks, Elizabeth A.;Fleming, Christopher D.;Mesecar, Andrew D.;Whitman, Christian P.;Pegan, Scott D.
通讯作者：
Pegan, Scott D.
影响因子：
2.9
作者：
Almrud, JJ;Kern, AD;Whitman, CP
通讯作者：
Whitman, CP
影响因子：
2.9
作者：
Czerwinski, RM;Harris, TK;Whitman, CP
通讯作者：
Whitman, CP
影响因子：
2.9
作者：
Czerwinski, RM;Harris, TK;Whitman, CP
通讯作者：
Whitman, CP
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC