Rice transcriptome analysis to identify possible herbicide quinclorac detoxification genes.
Rice transcriptome analysis to identify possible herbicide quinclorac detoxification genes.
复制标题
水稻转录组分析鉴定可能的除草剂二氯喹啉酸解毒基因
DOI：
10.3389/fgene.2015.00306
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发表时间：
2015
影响因子：
3.7
通讯作者：
Su Z
中科院分区：
文献类型：
--
作者：
Xu W;Di C;Zhou S;Liu J;Li L;Liu F;Yang X;Ling Y;Su Z
二氯喹啉酸是一种高选择性的生长素类除草剂，广泛用于有效控制稻田稗草，提高世界水稻产量。已经提出了二氯喹啉酸的除草剂作用模式，并且已经确定了影响二氯喹啉酸信号传导的激素相互作用。由于广泛使用，二氯喹啉酸可能随排水沃茨迁移到稻田外，导致土壤和水体污染等环境卫生问题。本研究利用57K亲和水稻全基因组芯片筛选二氯喹啉酸信号转导应答基因，研究二氯喹啉酸在水稻中的作用和解毒的分子机制。总体而言，637个探针集被确定为在二氯喹啉酸处理6或24小时下具有差异表达水平。生长素相关基因如GH3和OsIAAs对二氯喹啉酸处理有反应。Gene Ontology分析表明，解毒相关家族基因的基因显著富集，包括细胞色素P450、GST、UGT、ABC和药物转运蛋白基因。实时荧光定量PCR分析表明，CYP81、CYP709C和CYP72A等P450家族的候选基因普遍受到不同除草剂的诱导。在二氯喹啉酸处理下，同一P450家族的一些拟南芥基因表达上调。我们进行了水稻全基因组基因芯片分析，并首次在全球范围内鉴定了二氯喹啉酸反应基因。本研究为二氯喹啉酸的解毒和环境化学污染的生物监测提供了潜在的标志物。
Quinclorac is a highly selective auxin-type herbicide and is widely used in the effective control of barnyard grass in paddy rice fields, improving the world's rice yield. The herbicide mode of action of quinclorac has been proposed, and hormone interactions affecting quinclorac signaling has been identified. Because of widespread use, quinclorac may be transported outside rice fields with the drainage waters, leading to soil and water pollution and other environmental health problems. In this study, we used 57K Affymetrix rice whole-genome array to identify quinclorac signaling response genes to study the molecular mechanisms of action and detoxification of quinclorac in rice plants. Overall, 637 probe sets were identified with differential expression levels under either 6 or 24 h of quinclorac treatment. Auxin-related genes such as GH3 and OsIAAs responded to quinclorac treatment. Gene Ontology analysis showed that genes of detoxification-related family genes were significantly enriched, including cytochrome P450, GST, UGT, and ABC and drug transporter genes. Moreover, real-time RT-PCR analysis showed that top candidate genes of P450 families such as CYP81, CYP709C, and CYP72A were universally induced by different herbicides. Some Arabidopsis genes of the same P450 family were up-regulated under quinclorac treatment. We conducted rice whole-genome GeneChip analysis and the first global identification of quinclorac response genes. This work may provide potential markers for detoxification of quinclorac and biomonitors of environmental chemical pollution.