DNA synthesis technologies to close the gene writing gap.
DNA synthesis technologies to close the gene writing gap.
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DOI：
10.1038/s41570-022-00456-9
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发表时间：
2023
影响因子：
36.3
通讯作者：
Ryadnov, Maxim G
中科院分区：
文献类型：
--
作者：
Hoose, Alex;Vellacott, Richard;Storch, Marko;Freemont, Paul S;Ryadnov, Maxim G
合成DNA在许多研究和商业活动领域的需求日益增加。如果DNA能够大规模和低成本地提供，工程生物学、治疗、数据存储和纳米技术将会迅速发展。受下一代测序和基因编辑技术成功的刺激，DNA合成已经成为一个新兴产业。然而，>200 bp序列的合成仍然难以负担。为了克服这些限制，并开始像读取DNA一样有效地编写DNA，已经开发了替代技术，包括分子组装和克隆方法，不依赖模板的酶合成，微阵列和滚圈扩增技术。在这里，我们回顾了这些技术的开发和商业化进展，这些进展以领先公司的创新为例。我们讨论了每种技术的利弊，对DNA合成的整体监督和监管政策的需求，并概述了DNA合成的商业模式。对合成DNA的需求越来越大。然而，我们制造或写入DNA的能力落后于我们测序或读取DNA的能力。这篇综述讨论了商业化的DNA合成技术，以缩小DNA书写的差距。
Synthetic DNA is of increasing demand across many sectors of research and commercial activities. Engineering biology, therapy, data storage and nanotechnology are set for rapid developments if DNA can be provided at scale and low cost. Stimulated by successes in next generation sequencing and gene editing technologies, DNA synthesis is already a burgeoning industry. However, the synthesis of >200 bp sequences remains unaffordable. To overcome these limitations and start writing DNA as effectively as it is read, alternative technologies have been developed including molecular assembly and cloning methods, template-independent enzymatic synthesis, microarray and rolling circle amplification techniques. Here, we review the progress in developing and commercializing these technologies, which are exemplified by innovations from leading companies. We discuss pros and cons of each technology, the need for oversight and regulatory policies for DNA synthesis as a whole and give an overview of DNA synthesis business models. There is increasing demand for synthetic DNA. However, our ability to make, or write, DNA lags behind our ability to sequence, or read, it. This Review discusses commercialized DNA synthesis technologies in the pursuit of closing the DNA writing gap.
DOI：
10.4161/hv.4.6.6177
发表时间：
2008-11
期刊：
HUMAN VACCINES
影响因子：
--
作者：
Kendirgi, Frederic;Yun, Nadezda E.;Linde, Nathaniel S.;Zacks, Michele A.;Smith, Jeanon N.;Smith, Jennifer K.;McMicken, Harilyn;Chen, Yin;Paessler, Slobodan
通讯作者：
Paessler, Slobodan