Ascorbic acid and dehydroascorbic acid as biomarkers of oxidative stress caused by smoking
Ascorbic acid and dehydroascorbic acid as biomarkers of oxidative stress caused by smoking
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DOI：
10.1093/ajcn/65.4.959
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发表时间：
1997-04-01
影响因子：
7.1
通讯作者：
Poulsen, HE
中科院分区：
文献类型：
--
作者：
Lykkesfeldt, J;Loft, S;Poulsen, HE
使用可靠的，新开发的测定血浆中抗坏血酸（还原形式）和脱氢抗坏血酸（DHAA；氧化形式）的方法，我们研究了年龄，性别和吸烟对219名健康，年龄分层，随机选择的丹麦人口的影响。吸烟者血浆总抗坏血酸（抗坏血酸+ DHAA）浓度平均值（+/- SD） （62.8 +/- 24.9 mu mol/L）低于非吸烟者（74.9 +/- 23.6 mu mol/L） （P < 0.001）， DHAA含量为吸烟者总抗坏血酸的1.8 +/- 4.0%，而非吸烟者为0.1 +/- 3.1% （P < 0.001）。吸烟者DHAA含量与总抗坏血酸浓度呈显著负相关（P < 0.002），而吸烟者无显著负相关；两组线性回归斜率差异有统计学意义（P < 0.005）。女性总抗坏血酸平均血药浓度高于男性（P < 0.005）；在多变量分析中，当吸烟因素被调整后，这种差异仍然存在。没有年龄依赖性。数据表明，吸烟导致严重的氧化应激，消耗抗坏血酸池，并没有足够的还原能力来维持血浆中抗坏血酸的还原形式。我们认为，对dha的额外分析可以进一步区分氧化应激的评估，并可能提供一种确定吸烟者维生素C需求的客观方法。初步研究结果表明，吸烟者需要的维生素C剂量使其血浆浓度接近70 μ mol/L或更高。
Using a reliable, newly developed assay for ascorbic acid (reduced form) and dehydroascorbic acid (DHAA; the oxidized form) in plasma, we studied the influence of age, sex, and smoking on 219 healthy, age-stratified, and randomly selected subjects representing the Danish population. The mean (+/- SD) plasma total ascorbic acid (ascorbic acid + DHAA) concentration was lower in smokers (62.8 +/- 24.9 mu mol/L) than in nonsmokers (74.9 +/- 23.6 mu mol/L) (P < 0.001) and the DHAA content was 1.8 +/- 4.0% of the total ascorbic acid in smokers compared with 0.1 +/- 3.1% in nonsmokers (P < 0.001). A significant inverse correlation between the DHAA fraction and the total ascorbic acid concentration was found in smokers (P < 0.002) but not in smokers; the slopes of the linear regressions were significantly different in the two groups (P < 0.005). The mean plasma concentration of total ascorbic acid was higher in females than in males (P < 0.005); this difference persisted in multivariate analysis when smoking was adjusted for. No age dependence could be identified. The data show that smoking results in severe oxidative stress, depletion of the ascorbic acid pool, and insufficient reduction capacity to maintain ascorbic acid in the reduced form in plasma. We suggest that the additional analysis of DHAA allows further differentiation in the assessment of oxidative stress and may provide an objective way of determining vitamin C requirements in smokers. Preliminary findings suggest that a vitamin C dose that results in a plasma concentration of approximate to 70 mu mol/L or higher is required in smokers.