EXCITATORY SYNAPTIC TRANSMISSION IN CULTURES OF RAT OLFACTORY-BULB
EXCITATORY SYNAPTIC TRANSMISSION IN CULTURES OF RAT OLFACTORY-BULB
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DOI：
10.1152/jn.1990.64.2.598
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发表时间：
1990-08-01
影响因子：
2.5
通讯作者：
WESTBROOK, GL
中科院分区：
文献类型：
--
作者：
TROMBLEY, PQ;WESTBROOK, GL
1.嗅球神经元从新生大鼠中分离，并以低密度接种在嗅球星形胶质细胞的汇合层上。细胞内刺激假定的二尖瓣/簇状（M/T）细胞诱发单突触兴奋性突触后电位（EPSP）在相邻的神经元。全细胞记录技术和流管给药系统被用来比较EPSP与电压钳记录的电流诱发兴奋性氨基酸（EAA），包括N-乙酰基谷氨酸（NAAG），一个假定的二尖瓣细胞递质。2.培养的嗅球神经元在形态和生理上是不同的。大的杯形神经元，这是NAAG免疫反应，这些神经元的刺激总是诱发EPSP，这表明他们是M/T细胞。大多数小的双极神经元谷氨酸脱羧酶（GAD）免疫反应一致的颗粒或肾小球周围的γ-氨基丁酸（GABA）能中间神经元。3. M/T细胞间的单突触EPSP可被EAA受体拮抗剂分为快、慢两种成分。非NMDA受体拮抗剂6-氰基-2，3-二羟基-7-硝基-喹喔啉（CNQX，2.5 μ M）可阻断快速组分，其达峰时间为7.7 +/- 1.0（SE）ms，半宽为31.8 +/- 7.4 ms。慢成分（达峰时间= 41.4 +/- 7.2 ms;半宽= 218.9 +/- 40.4 ms）被N-甲基-D-天冬氨酸（NMDA）受体拮抗剂DL-2-氨基-5-膦酰基戊酸（AP 5，100 μ M）阻断。4.在电压钳下，在无镁溶液中，在-60 mV的保持电位下，流管应用NAAG（10- 1，000 μ M）诱发内向电流。（250字处删节）
1. Olfactory bulb neurons were dissociated from neonatal rats and plated at low density on a confluent layer of olfactory bulb astrocytes. Intracellular stimulation of presumptive mitral/tufted (M/T) cells evoked monosynaptic excitatory postsynaptic potentials (EPSPs) in adjacent neurons. Whole-cell recording techniques and a flow-pipe drug delivery system were used to compare EPSPs with voltage-clamp recordings of currents evoked by excitatory amino acids (EAA) including N-acetylaspartylglutamate (NAAG), a putative mitral cell transmitter. 2. Cultured olfactory bulb neurons were morphologically and physiologically distinct. Large pyramidal-shaped neurons were present, which were NAAG immunoreactive; stimulation of these neurons invariably evoked EPSPs, suggesting that they were M/T cells. The majority of small bipolar neurons were glutamic acid decarboxylase (GAD) immunoreactive consistent with granule or periglomerular gamma-aminobutyric acid (GABA)ergic interneurons. 3. Monosynaptic EPSPs between M/T cells could be separated into fast and slow components by the use of EAA receptor antagonists. A fast component with a time-to-peak of 7.7 +/- 1.0 (SE) ms and half-width of 31.8 +/- 7.4 ms was blocked by the non-NMDA receptor antagonist 6-cyano-2,3-dihydroxy-7-nitro-quinoxaline (CNQX, 2.5 microM). The slow component (time-to-peak = 41.4 +/- 7.2 ms; half-width = 218.9 +/- 40.4 ms) was blocked by the N-methyl-D-aspartate (NMDA) receptor antagonist DL-2-amino-5-phosphonovaleric acid (AP5, 100 microM). 4. Under voltage clamp, flow-pipe applications of NAAG (10-1,000 microM) evoked inward currents at a holding potential of -60 mV in Mg-free solutions.(ABSTRACT TRUNCATED AT 250 WORDS)