Early flora colonization affects intestinal immunoglobulin G uptake in piglets, which may be mediated by NF-κB-FcRn pathway.
Early flora colonization affects intestinal immunoglobulin G uptake in piglets, which may be mediated by NF-κB-FcRn pathway.
复制标题
早期菌群定植影响仔猪肠道免疫球蛋白 G 的摄取，这可能是由 NF-κB-FcRn 通路介导的
DOI：
10.3389/fmicb.2023.1136513
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
新生仔猪的被动免疫主要来源于母乳中的免疫球蛋白G（IgG），被动免疫传递不完全被认为是仔猪死亡的重要原因。本研究旨在探讨早期肠道植物群定植对IgG摄取的影响及其可能机制。以新生仔猪和IPEC-J2细胞为实验材料，研究了影响肠道IgG摄取的可能因素和调控机制。在体内，所有40头仔猪在出生后第0、1、3和7天处死，每次10头仔猪。收集血液样品、胃内容物、空肠内容物和粘膜进行分析。体外利用IPEC-J2细胞transwell培养系统建立IgG转运体模型，探讨IgG转运的特异性调控机制。我们的结果表明，肠道IgG摄取与新生儿Fc受体（FcRn）的表达呈正相关。随着日龄的增加，新生仔猪肠道植物群逐渐丰富。肠道基因的功能也随着肠道植物群的定植而发生变化。TLR 2、TLR 4和NF-κB（P65）在小肠中的表达趋势与FcRn的表达趋势一致。此外，体外实验结果表明NF-κB信号通路参与了FcRn介导的IgG跨膜转运的调节。早期植物群定植影响仔猪肠道IgG摄取，这可能是通过NF-κB-FcRn途径介导的。
The passive immunity of newborn piglets is mainly derived from immunoglobulin G (IgG) in breast milk, and the incomplete transfer of passive immune is considered to be an important cause of piglet death. This study was conducted to investigate the effect of early intestinal flora colonization on IgG uptake and its possible mechanism. The newborn piglets and IPEC-J2 cells were used to investigate the possible factors and regulatory mechanisms affecting intestinal IgG uptake. In vivo, all 40 piglets were euthanized on postnatal d 0, 1, 3, and 7, with 10 piglets per time. The blood sample, gastric contents, jejunal contents and mucosa were collected for analysis. In vitro, IPEC-J2 cells transwell culture system was used to establish the IgG transporter model to explore the specific regulatory mechanism of IgG transport. Our results demonstrated that the intestinal IgG uptake was positively correlated with the expression of Neonatal Fc receptor (FcRn). With the increase of age, the intestinal flora of newborn piglets was gradually enriched. The function of intestinal genes also changes with the colonization of intestinal flora. We found that the expression trend of TLR2, TLR4 and NF-κB (P65) in intestine was consistent with that of FcRn. Furthermore, the in vitro results demonstrate that the NF-κB signaling pathway is involved in regulating FcRn-mediated IgG transmembrane transport. Early flora colonization affects intestinal IgG uptake in piglets, which may be mediated by NF-κB-FcRn pathway.