A Label-Free Nanogold DNAzyme-Cleaved Surface-Enhanced Resonance Raman Scattering Method for Trace UO22+ Using Rhodamine 6G as Probe
A Label-Free Nanogold DNAzyme-Cleaved Surface-Enhanced Resonance Raman Scattering Method for Trace UO22+ Using Rhodamine 6G as Probe
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以罗丹明 6G 为探针的无标记纳米金脱氧核糖核酸酶切割表面增强共振拉曼散射法测定痕量 UO22
DOI：
10.1007/s11468-012-9476-8
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发表时间：
2013-06-01
期刊：
影响因子：
3
通讯作者：
Liang, Aihui
中科院分区：
文献类型：
--
作者：
Jiang, Zhiliang;Yao, Dongmei;Liang, Aihui
在 80 ℃、pH5.5 含有 0.25 M NaCl 的 2-(N-吗啉代)-乙酰磺酸缓冲溶液中，单链底物 DNA 与酶 DNA 杂交形成双链 DNA (dsDNA)。双链DNA的底物链可以被UO22+催化裂解，产生短单链DNA（ssDNA），该短单链DNA（ssDNA）吸附在纳米金（NG）表面上，形成稳定的纳米金-ssDNA缀合物，然后进一步与罗丹明6G（RhG）结合形成NG-ssDNA-RhG缀合物，可以通过表面增强共振拉曼散射（SERRS）光谱技术进行监测1,360 厘米(-1)。在所选条件下，增加的SERRS强度Delta I-1360与UO22+浓度在5-125 nmol/L范围内呈线性关系，检测限为1.6 nmol/L。使用0.5-mu mol/L Hg2+作为增强剂，可以测定2.5-100-nmol/L UO22+。
In pH5.5 2-(N-morpholino)-ethanosulfonic acid buffer solution containing 0.25 M NaCl at 80 degrees C, the single-stranded substrate DNA hybridizes with the enzyme DNA to form double-stranded DNA (dsDNA). The substrate chain of dsDNA could be cracked catalytically by UO22+ to produce a short single-stranded DNA (ssDNA) that adsorbed on the nanogold (NG) surface to form a stable nanogold-ssDNA conjugate and then further combine with rhodamine 6G (RhG) to form a NG-ssDNA-RhG conjugate that can be monitored by the surface-enhanced resonance Raman scattering (SERRS) spectral technique at 1,360 cm(-1). Under the selected conditions, the increased SERRS intensity Delta I-1360 was linear to UO22+ concentration in the range of 5-125 nmol/L, with a detection limit of 1.6 nmol/L. Using a 0.5-mu mol/L Hg2+ as enhancer, a 2.5-100-nmol/L UO22+ can be determined.